NG2 Antibody (132.38): sc-33666

NG2 Antibody (132.38) is a mouse monoclonal IgG1 kappa light chain antibody that detects NG2 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-NG2 antibody (132.38) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NG2, also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16, and MEL-CSPG, plays a crucial role in stabilizing cell-substratum interactions during the early stages of melanoma cell spreading on endothelial basement membranes, which is vital for tumor progression. NG2 is primarily located on the surface of various cell types, including blood vessels in the rat embryo and endothelial cells in the central nervous system, where NG2 contributes to the structural integrity and functionality of microvessels. Additionally, NG2 is expressed on smooth muscle cells outside the CNS and serves as a novel marker for epidermal stem cells, promoting their clustering and patterned distribution. Notably, the phosphorylation of threonine 2256 in rat NG2 (threonine 2252 in human NG2) leads to redistribution on astrocytoma surfaces, enhancing cell polarization and motility, which are critical factors in cancer metastasis. NG2 also acts as a co-receptor for α4 β1 integrin, facilitating spreading and focal contact formation in malignant melanoma cells, thereby underscoring its importance in cancer biology and potential therapeutic targeting.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NG2 Antibody (132.38) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-NG2 Antibody (132.38) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NG2 Antibody (132.38) Specifications

Alternate Product Name: NG2

Product Clone Name: 132.38

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1592-2222

Epitope Target: NG2

Epitope Species: rat

Product Reactivity/Detection: NG2

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for NG2 Antibody (132.38): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NG2 antibody is NG2 Antibody (LHM 2): sc-53389.