NFRκB Double Nickase Plasmid (h): sc-407203-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFRκB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFRκB Double Nickase Plasmid (h) and NFRκB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFRKB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFRκB Antibody (A-12): sc-514977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFRκB Double Nickase Plasmid (h) | sc-407203-NIC | 20 µg | $410.00 | |||
NFRκB Double Nickase Plasmid (h2) | sc-407203-NIC-2 | 20 µg | $410.00 |
NFRKB (nuclear factor related to kappa-B binding protein) encodes a DNA-binding factor originally identified through interactions with NF-κB regulatory elements and is implicated in transcriptional control programs linked to chromatin organization. The protein has been reported as a component associated with ATP-dependent chromatin remodeling complexes, supporting roles in gene expression regulation, genome maintenance, and cell-cycle–coupled nuclear processes. Through connections to NF-κB–adjacent transcriptional circuitry, NFRKB is studied in contexts where inflammatory signaling intersects with nuclear remodeling and stress responses. Dysregulated chromatin regulation and NF-κB–related transcriptional outputs are frequently explored in cancer biology and immune-associated disease models, making NFRKB a useful node for mechanistic studies of transcription and nuclear regulation.
NFRκB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFRKB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFRKB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFRKB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFRKB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.