NFκB p50 (h): 293T Lysate: sc-116112

NFκB p50 (h): 293T Lysate, derived from the HEK 293T cell line, is a crucial resource for studying the NFκB p50 subunit, a key transcription factor involved in regulating immune responses, inflammation, and cell survival. The HEK 293T cells, a variant of the original HEK 293 cells, are engineered to express the SV40 large T antigen, enhancing their capacity for high-efficiency protein production, making them ideal for generating lysates rich in NFκB p50. Researchers utilize NFκB p50 (h): 293T Lysate to investigate the mechanisms by which NFκB p50 regulates gene expression. NFκB p50, typically part of the NFκB heterodimer (p50/p65), translocates to the nucleus in response to various stimuli, including cytokines, stress, and microbial infections. Upon nuclear translocation, NFκB p50 binds to specific DNA sequences known as κB sites, modulating the transcription of target genes involved in inflammatory and immune responses. Techniques such as chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and reporter gene assays are employed to study the DNA-binding activity of NFκB p50 and its interaction with co-factors and inhibitors. This lysate is instrumental in elucidating the fundamental mechanisms of NFκB signaling pathways, providing insights into the regulation of gene expression during immune and inflammatory responses. By focusing on basic biological research, the use of NFκB p50 (h): 293T Lysate allows scientists to understand the molecular dynamics and regulatory mechanisms of NFκB p50.