NFATc4 (h): 293T Lysate: sc-116481
- human NFATc4 transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for NFATc4 antibodies
- Suitable control antibody: NFATc4 Antibody (B-2): sc-271597 is recommended as control antibody for monitoring of NFATc4 expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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NFATc4 (h): 293T Lysate, derived from the HEK 293T cell line, is essential for investigating the Nuclear Factor of Activated T-cells, cytoplasmic 4 (NFATc4) protein, a crucial transcription factor in cellular signaling pathways. The HEK 293T cells, an enhanced version of the original HEK 293 cells, express the SV40 large T antigen, which significantly boosts protein production, making these lysates particularly useful for detailed molecular studies. Researchers utilize NFATc4 (h): 293T Lysate to explore the mechanisms by which NFATc4 translocates to the nucleus upon dephosphorylation by calcineurin in response to calcium signaling. This translocation enables NFATc4 to bind to specific DNA sequences, regulating the expression of genes involved in various cellular processes, including development, differentiation, and immune responses. Techniques such as chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and reporter gene assays are commonly employed to study NFATc4's DNA-binding activity and its interaction with other transcription factors. This lysate is instrumental in understanding the role of NFATc4 in non-medical contexts, such as its involvement in the regulation of gene expression during cellular activation and its impact on intracellular signaling networks.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFATc4 (h): 293T Lysate | sc-116481 | 100 µg/200 µl | $232.00 |