NFATc4 Antibody (B-2): sc-271597

NFATc4 (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects NFATc4 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). NFATc4 (B-2) antibody is available as non-conjugated form, as well as multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 4) is a member of the nuclear factors of activated T cells DNA-binding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. This complex consists of at least two components: a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4.

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NFATc4 Antibody (B-2) References:

1. Requirement of two NFATc4 transactivation domains for CBP potentiation.  |  Yang, T., et al. 2001. J Biol Chem. 276: 39569-76. PMID: 11514544
2. Phosphorylation of NFATc4 by p38 mitogen-activated protein kinases.  |  Yang, TT., et al. 2002. Mol Cell Biol. 22: 3892-904. PMID: 11997522
3. Targeted disruption of NFATc3, but not NFATc4, reveals an intrinsic defect in calcineurin-mediated cardiac hypertrophic growth.  |  Wilkins, BJ., et al. 2002. Mol Cell Biol. 22: 7603-13. PMID: 12370307
4. Neurotrophins and netrins require calcineurin/NFAT signaling to stimulate outgrowth of embryonic axons.  |  Graef, IA., et al. 2003. Cell. 113: 657-70. PMID: 12787506
5. A ternary complex of transcription factors, Nishéd and NFATc4, and co-activator p300 bound to an intronic sequence, intronic regulatory element, is pivotal for the up-regulation of myosin light chain-2v gene in cardiac hypertrophy.  |  Mathew, S., et al. 2004. J Biol Chem. 279: 41018-27. PMID: 15272022
6. Calcineurin/NFAT-induced up-regulation of the Fas ligand/Fas death pathway is involved in methamphetamine-induced neuronal apoptosis.  |  Jayanthi, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 868-73. PMID: 15644446
7. Recruitment of the extracellular signal-regulated kinase/ribosomal S6 kinase signaling pathway to the NFATc4 transcription activation complex.  |  Yang, TT., et al. 2005. Mol Cell Biol. 25: 907-20. PMID: 15657420
8. Regulation of calcineurin through transcriptional induction of the calcineurin A beta promoter in vitro and in vivo.  |  Oka, T., et al. 2005. Mol Cell Biol. 25: 6649-59. PMID: 16024800
9. Immunolocalization of NFATc4 in the adult mouse brain.  |  Bradley, KC., et al. 2005. J Neurosci Res. 82: 762-70. PMID: 16273547
10. Nuclear factor of activated T-cells isoform c4 (NFATc4/NFAT3) as a mediator of antiapoptotic transcription in NMDA receptor-stimulated cortical neurons.  |  Vashishta, A., et al. 2009. J Neurosci. 29: 15331-40. PMID: 19955386
11. Nuclear factor of activated T cells (NFATc4) is required for BDNF-dependent survival of adult-born neurons and spatial memory formation in the hippocampus.  |  Quadrato, G., et al. 2012. Proc Natl Acad Sci U S A. 109: E1499-508. PMID: 22586092
12. Ethanol inhibition of undifferentiated rat neural progenitor cell replication can be prevented by chlorogenic acid via the NFATc4/CSE signaling pathway.  |  Shanmugam, S., et al. 2023. Alcohol Clin Exp Res (Hoboken). 47: 1530-1543. PMID: 37364904

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NFATc4 Antibody (B-2) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-NFATc4 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NFATc4 Antibody (B-2) Specifications

Alternate Product Name: NFATc4

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 125-198

Epitope Target: NFATc4

Epitope Species: human

Product Reactivity/Detection: NFATc4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for NFATc4 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent