Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NFATc1 CRISPR/Cas9 KO Plasmid (h): sc-400164
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFATc1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NFATc1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFATc1 Antibody (7A6): sc-7294
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFATc1 CRISPR/Cas9 KO Plasmid (h) | sc-400164 | 20 µg | $397.00 |
Overview
NFATC1 encodes NFATc1, a calcium/calcineurin-regulated transcription factor that translocates to the nucleus following T cell receptor signaling to coordinate immune activation programs. NFATc1 integrates Ca2+-dependent signaling with MAPK and AP-1 inputs to control transcription of cytokines, co-stimulatory molecules, and differentiation-associated genes in lymphocytes. Beyond immunity, NFATc1 contributes to osteoclastogenesis and cell fate decisions through crosstalk with RANKL, NF-κB, and other transcriptional networks. Dysregulated NFATc1 activity has been associated with inflammatory pathobiology and aberrant transcriptional states observed across multiple cancer-related contexts, supporting its use in mechanistic studies of signaling-to-transcription coupling.
NFATc1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NFATC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NFATC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NFATC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NFATc1 protein expression.
This CRISPR knockout system enables efficient generation of NFATC1-deficient cell models for investigation of NFATc1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NFATC1 exon(s) critical for NFATc1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NFATC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NFATc1 CRISPR/Cas9 KO Plasmid (h) and NFATc1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NFATC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NFATc1 HDR Plasmid (h) and NFATc1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NFATC1 homology arms to support homology-directed repair at defined NFATC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.