Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NFAT5 CRISPR/Cas9 KO Plasmid (h): sc-401359
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFAT5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NFAT5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFAT5 Antibody (F-9): sc-398171
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFAT5 CRISPR/Cas9 KO Plasmid (h) | sc-401359 | 20 µg | $397.00 |
Overview
NFAT5 (nuclear factor of activated T cells 5), also known as TonEBP, is a Rel-family transcription factor that coordinates cellular adaptation to hypertonic stress by regulating osmoprotective gene programs, including transporters and enzymes involved in compatible osmolyte accumulation. In immune and epithelial contexts, NFAT5 influences cytokine production, macrophage polarization, and lymphocyte function, linking osmotic sensing to inflammatory signaling and cellular survival pathways. NFAT5 activity intersects with MAPK and calcium-dependent signaling networks and helps maintain protein homeostasis and cell volume control under stress. Dysregulated NFAT5 signaling has been associated with inflammatory disorders, renal medullary physiology, and tumor microenvironment stress responses, making it relevant for mechanistic studies of stress adaptation and immune regulation.
NFAT5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NFAT5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NFAT5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NFAT5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NFAT5 protein expression.
This CRISPR knockout system enables efficient generation of NFAT5-deficient cell models for investigation of NFAT5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NFAT5 exon(s) critical for NFAT5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NFAT5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NFAT5 CRISPR/Cas9 KO Plasmid (h) and NFAT5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NFAT5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NFAT5 HDR Plasmid (h) and NFAT5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NFAT5 homology arms to support homology-directed repair at defined NFAT5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.