NF-YA Antibody (G-2): sc-17753

NF-YA Antibody (G-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects NF-YA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-NF-YA antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NF-YA plays a crucial role in gene regulation as a component of the CCAAT-binding factor NF-Y, which is a heteromeric transcription factor essential for binding to CCAAT sequences in numerous eukaryotic genes. NF-YA (G-2) antibody targets a protein that forms a stable complex with NF-YB and NF-YC, which is necessary for effective DNA binding and transcriptional activation. This complex is highly conserved across species, with the DNA binding domains of NF-YB and NF-YC exhibiting a unique protein-protein interaction through a histone-fold "handshake" motif, reminiscent of the interactions seen in histone proteins. Such structural conservation underscores the fundamental role of NF-YA in regulating gene expression, making NF-YA an important target for studies related to cellular growth, differentiation, and response to environmental signals.

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NF-YA Antibody (G-2) References:

1. NF-YA overexpression protects from glutamine deprivation.  |  Dolfini, D., et al. 2020. Biochim Biophys Acta Mol Cell Res. 1867: 118571. PMID: 31706909
2. NF-YA Overexpression in Lung Cancer: LUAD.  |  Bezzecchi, E., et al. 2020. Genes (Basel). 11: PMID: 32075093
3. Metabolic Syndrome Mediates ROS-miR-193b-NFYA-Dependent Downregulation of Soluble Guanylate Cyclase and Contributes to Exercise-Induced Pulmonary Hypertension in Heart Failure With Preserved Ejection Fraction.  |  Satoh, T., et al. 2021. Circulation. 144: 615-637. PMID: 34157861
4. CDCA8 induced by NF-YA promotes hepatocellular carcinoma progression by regulating the MEK/ERK pathway.  |  Chen, E., et al. 2023. Exp Hematol Oncol. 12: 9. PMID: 36639822
5. NF-YA isoforms with alternative splicing of exon-5 in Aves.  |  Gallo, A., et al. 2023. Genomics. 115: 110694. PMID: 37536396
6. Transcription factor NFYa controls cardiomyocyte metabolism and proliferation during mouse fetal heart development.  |  Cui, M., et al. 2023. Dev Cell. 58: 2867-2880.e7. PMID: 37972593
7. A variety of DNA-binding and multimeric proteins contain the histone fold motif.  |  Baxevanis, AD., et al. 1995. Nucleic Acids Res. 23: 2685-91. PMID: 7651829
8. Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex.  |  Sinha, S., et al. 1996. Mol Cell Biol. 16: 328-37. PMID: 8524312
9. Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y).  |  Currie, RA. 1997. J Biol Chem. 272: 30880-8. PMID: 9388234
10. A survey of 178 NF-Y binding CCAAT boxes.  |  Mantovani, R. 1998. Nucleic Acids Res. 26: 1135-43. PMID: 9469818
11. Role of the CCAAT-binding protein CBF/NF-Y in transcription.  |  Maity, SN. and de Crombrugghe, B. 1998. Trends Biochem Sci. 23: 174-8. PMID: 9612081
12. Pathway of complex formation between DNA and three subunits of CBF/NF-Y. Photocross-linking analysis of DNA-protein interaction and characterization of equilibrium steps of subunit interaction and dna binding.  |  Liang, SG. and Maity, SN. 1998. J Biol Chem. 273: 31590-8. PMID: 9813075

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NF-YA Antibody (G-2) has 68 citations in a variety of scientific publications.

Primary antibodies like Anti-NF-YA Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NF-YA Antibody (G-2) Specifications

Alternate Product Name: Nuclear transcription factor Y subunit alpha

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 139-347

Epitope Target: NF-YA

Epitope Species: human

Product Reactivity/Detection: NF-YA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 68 citations in various scientific publications

Available Conjugates/Forms for NF-YA Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent