NF-M Antibody (3H11): sc-58561

NF-M Antibody (3H11) is a mouse monoclonal IgG1 antibody that detects NF-M from mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-NF-M antibody (3H11) is available in non-conjugated form. Neurofilament-M (NF-M), a key member of the intermediate filament family, plays a crucial role in maintaining neuronal cell structure by forming a major component of the neuronal cytoskeleton. NF-M′s structure consists of a central rod domain flanked by head and tail domains, allowing formation of heteropolymers with neurofilament light and heavy chains, contributing to axonal stability and resilience. This structural arrangement supports axonal caliber and facilitates organelle and protein transport along axons, vital for neuronal function. Post-translational modifications of NF-M, such as phosphorylation, can lead to significant changes in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, highlighting NF-M′s importance in health and disease.

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NF-M Antibody (3H11) References:

1. Activation of mitogen-activated protein kinases (Erk1 and Erk2) cascade results in phosphorylation of NF-M tail domains in transfected NIH 3T3 cells.  |  Li, BS., et al. 1999. Eur J Biochem. 262: 211-7. PMID: 10231383
2. Casein kinase II is responsible for phosphorylation of NF-L at Ser-473.  |  Nakamura, Y., et al. 1999. FEBS Lett. 455: 83-6. PMID: 10428477
3. Neurofilament metabolism in sporadic amyotrophic lateral sclerosis.  |  Strong, MJ. 1999. J Neurol Sci. 169: 170-7. PMID: 10540027
4. Effects of phosphorylation of the neurofilament L protein on filamentous structures.  |  Hisanaga, S., et al. 1990. Cell Regul. 1: 237-48. PMID: 2100199
5. Involvement of protein kinase C in the regulation of assembly-disassembly of neurofilaments in vitro.  |  Gonda, Y., et al. 1990. Biochem Biophys Res Commun. 167: 1316-25. PMID: 2108674
6. In vivo phosphorylation of distinct domains of the 70-kilodalton neurofilament subunit involves different protein kinases.  |  Sihag, RK. and Nixon, RA. 1989. J Biol Chem. 264: 457-64. PMID: 2491851
7. Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures.  |  Angelides, KJ., et al. 1989. J Cell Biol. 108: 1495-506. PMID: 2925792
8. Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.  |  Levy, E., et al. 1987. Eur J Biochem. 166: 71-7. PMID: 3036526
9. Cytoskeletal neurofilament gene expression in brain tissue from Alzheimer's disease patients. I. Decrease in NF-L and NF-M message.  |  Kittur, S., et al. 1994. J Geriatr Psychiatry Neurol. 7: 153-8. PMID: 7522458
10. Two distinct functions of the carboxyl-terminal tail domain of NF-M upon neurofilament assembly: cross-bridge formation and longitudinal elongation of filaments.  |  Nakagawa, T., et al. 1995. J Cell Biol. 129: 411-29. PMID: 7721944
11. Abnormal distribution of neurofilament L in neurons with Alzheimer's disease.  |  Nakamura, Y., et al. 1997. Neurosci Lett. 225: 201-4. PMID: 9147405
12. Antagonistic roles of neurofilament subunits NF-H and NF-M against NF-L in shaping dendritic arborization in spinal motor neurons.  |  Kong, J., et al. 1998. J Cell Biol. 140: 1167-76. PMID: 9490729

The preferred NF-M antibody is NF-M Antibody (E-9): sc-398532.