NFκB p50 Double Nickase Plasmid (h): sc-400087-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFκB p50 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFκB p50 Double Nickase Plasmid (h) and NFκB p50 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFKB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFκB p50 Antibody (E-10): sc-8414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFκB p50 Double Nickase Plasmid (h) | sc-400087-NIC | 20 µg | $410.00 | |||
NFκB p50 Double Nickase Plasmid (h2) | sc-400087-NIC-2 | 20 µg | $410.00 |
NFKB1 encodes the NFκB p50 subunit, a central component of NF-κB transcription factor complexes that regulate inducible gene expression in response to inflammatory cytokines, Toll-like receptor signaling, antigen receptor engagement, and cellular stress. NFκB p50 is generated by proteolytic processing of the p105 precursor and forms homo- or heterodimers (commonly with RelA/p65) that control transcriptional programs governing innate and adaptive immunity, cell survival, and differentiation. Through canonical and non-canonical NF-κB pathways, p50 influences chromatin accessibility and cytokine/chemokine networks, shaping responses to infection and tissue injury. Dysregulated NFKB1 activity and pathway crosstalk with MAPK, JAK/STAT, and interferon signaling are frequently implicated in chronic inflammation, immune dysfunction, and oncogenic microenvironments.
NFκB p50 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFKB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFKB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFKB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFKB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.