NF-1C Antibody (4E10-D8-F4): sc-517555

NF-1C Antibody (4E10-D8-F4) is a mouse monoclonal IgG antibody that detects NF-1C protein of mouse, rat, and human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-NF-1C antibody (4E10-D8-F4) is available as a non-conjugated format. NF-1C protein, a member of the NF-1 family of CCAAT box-binding proteins, plays a crucial role in regulating gene expression by stimulating DNA replication and activating transcription. This 508 amino acid protein is localized in the nucleus and features a CTF/NF-I DNA-binding domain, allowing formation of homodimers that bind to specific DNA sequences, such as the palindromic sequence 5′-TTGGCNNNNNGCCAA-3′. Such binding influences transcriptional regulation of both cellular and viral promoters, thereby impacting various biological processes, including TGFβ signaling and extracellular matrix deposition. NF-1C is expressed in multiple tissues, including brain, liver, spleen, and heart, and exists in five alternatively spliced isoforms, highlighting diverse functional roles in cellular physiology and pathology.

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NF-1C Antibody (4E10-D8-F4) References:

1. CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription.  |  Leahy, P., et al. 1999. J Biol Chem. 274: 8813-22. PMID: 10085123
2. NF-1C, Sp1, and Sp3 are essential for transcription of the human gene for P450c17 (steroid 17alpha-hydroxylase/17,20 lyase) in human adrenal NCI-H295A cells.  |  Lin, CJ., et al. 2001. Mol Endocrinol. 15: 1277-93. PMID: 11463853
3. RFX1 and NF-1 associate with P sequences of the human growth hormone locus in pituitary chromatin.  |  Norquay, LD., et al. 2003. Mol Endocrinol. 17: 1027-38. PMID: 12624117
4. A role for nuclear factor I in the intrinsic control of cerebellar granule neuron gene expression.  |  Wang, W., et al. 2004. J Biol Chem. 279: 53491-7. PMID: 15466411
5. Transcriptional regulation of the human alpha6 integrin gene by the transcription factor NFI during corneal wound healing.  |  Gaudreault, M., et al. 2008. Invest Ophthalmol Vis Sci. 49: 3758-67. PMID: 18421093
6. Tissue- and cell-specific alternative splicing of NFIC.  |  Lamani, E., et al. 2009. Cells Tissues Organs. 189: 105-10. PMID: 18765927
7. Nuclear factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts.  |  Pérez-Casellas, LA., et al. 2009. Biochim Biophys Acta. 1789: 78-87. PMID: 18809517
8. Nuclear factor I-C links platelet-derived growth factor and transforming growth factor beta1 signaling to skin wound healing progression.  |  Plasari, G., et al. 2009. Mol Cell Biol. 29: 6006-17. PMID: 19752192
9. Inhibition of AHR transcription by NF1C is affected by a single-nucleotide polymorphism, and is involved in suppression of human uterine endometrial cancer.  |  Li, D., et al. 2013. Oncogene. 32: 4950-9. PMID: 23208493
10. Stilbenoid-Mediated Epigenetic Activation of Semaphorin 3A in Breast Cancer Cells Involves Changes in Dynamic Interactions of DNA with DNMT3A and NF1C Transcription Factor.  |  Beetch, M., et al. 2019. Mol Nutr Food Res. 63: e1801386. PMID: 31327173
11. Insulin-like growth factor 2 mRNA-binding protein 2-regulated alternative splicing of nuclear factor 1 C-type causes excessive granulosa cell proliferation in polycystic ovary syndrome.  |  Zhao, F., et al. 2022. Cell Prolif. 55: e13216. PMID: 35293050
12. Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH.  |  Qian, F., et al. 1995. Genomics. 28: 66-73. PMID: 7590749

The preferred NF-1 antibody is NF-1 Antibody (D-2): sc-74444.