Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neutrophil Elastase Double Nickase Plasmid (h): sc-400677-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neutrophil Elastase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Neutrophil Elastase Double Nickase Plasmid (h) and Neutrophil Elastase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ELANE. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neutrophil Elastase Antibody (G-2): sc-55549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neutrophil Elastase Double Nickase Plasmid (h) | sc-400677-NIC | 20 µg | $410.00 | |||
Neutrophil Elastase Double Nickase Plasmid (h2) | sc-400677-NIC-2 | 20 µg | $410.00 |
ELANE encodes neutrophil elastase, a serine protease stored in azurophilic granules that is released during degranulation and neutrophil extracellular trap formation to remodel extracellular matrix and degrade microbial proteins. Neutrophil elastase activity intersects with innate immune signaling, protease–antiprotease balance, and inflammatory proteolysis, influencing chemotaxis, cytokine processing, and tissue injury responses. Dysregulated ELANE function or expression is linked to neutrophil-driven inflammatory pathology and impaired host defense, and ELANE variants are associated with congenital neutropenia phenotypes. As a result, ELANE is widely studied in myeloid differentiation, granule biogenesis, and mechanisms that couple neutrophil activation to proteolytic microenvironments.
Neutrophil Elastase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ELANE locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ELANE. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ELANE function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ELANE-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.