Neurotensin Antibody (E-5): sc-377503

Neurotensin Antibody (E-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Neurotensin protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Neurotensin antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neurotensin precursor, also known as Pro-NT/NMN, is a 170 amino acid protein that undergoes cleavage at carboxy terminal dibasic residues by prohormone convertase, resulting in three processed peptides: Neurotensin (NT), Neuromedin N (NMN), and large Neuromedin N (NMN-125). Processed Neurotensin peptide, consisting of 13 amino acids, is primarily produced in the central nervous system and intestine, where Neurotensin plays a crucial role as a neurotransmitter that modulates dopaminergic signaling pathways in the brain. Additionally, Neurotensin acts as a parahormone that may influence the growth of human colon cancers, highlighting Neurotensin′s significance in both neurological functions and potential implications in cancer biology. Interaction of processed Neurotensin and Neuromedin N with G-protein coupled Neurotensin receptor (NTR) leads to hydrolysis of phosphatidylinositols and mobilization of calcium, further underscoring Neurotensin′s importance in cellular signaling pathways.

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Neurotensin Antibody (E-5) References:

1. Actions of neurotensin: a review of the electrophysiological studies.  |  Shi, WX. and Bunney, BS. 1992. Ann N Y Acad Sci. 668: 129-45. PMID: 1463269
2. Brain neurotensin, psychostimulants, and stress--emphasis on neuroanatomical substrates.  |  Geisler, S., et al. 2006. Peptides. 27: 2364-84. PMID: 16934369
3. The rat gene encoding neurotensin and neuromedin N. Structure, tissue-specific expression, and evolution of exon sequences.  |  Kislauskis, E., et al. 1988. J Biol Chem. 263: 4963-8. PMID: 2832414
4. Oncogenic role of neurotensin and neurotensin receptors in various cancers.  |  Ouyang, Q., et al. 2017. Clin Exp Pharmacol Physiol. 44: 841-846. PMID: 28556374
5. Biasing Neurotensin Receptor Signaling to Arrest Psychostimulant Abuse.  |  Dobbs, LK. and Morikawa, H. 2020. Cell. 181: 1205-1206. PMID: 32470394
6. Neurotensin and Neurotensin Receptors in Stress-related Disorders: Pathophysiology & Novel Drug Targets.  |  Kyriatzis, G., et al. 2024. Curr Neuropharmacol. 22: 916-934. PMID: 37534788
7. Neurotensin modulates ovarian vascular permeability via adherens junctions.  |  Pearson, AC., et al. 2024. FASEB J. 38: e23602. PMID: 38581236
8. Post-translational processing of the neurotensin/neuromedin N precursor in the central nervous system of the rat--I. Biochemical characterization of maturation products.  |  de Nadai, F., et al. 1994. Neuroscience. 60: 159-66. PMID: 8052409
9. Biosynthesis and posttranslational processing of the neurotensin/neuromedin N precursor in the rat medullary thyroid carcinoma 6-23 cell line. Effect of dexamethasone.  |  de Nadai, F., et al. 1993. Endocrinology. 132: 1614-20. PMID: 8462460
10. Neurotensin receptors: binding properties, transduction pathways, and structure.  |  Vincent, JP. 1995. Cell Mol Neurobiol. 15: 501-12. PMID: 8719037
11. Pro-neurotensin/neuromedin N expression and processing in human colon cancer cell lines.  |  Rovère, C., et al. 1998. Biochem Biophys Res Commun. 246: 155-9. PMID: 9600085
12. PC5-A-mediated processing of pro-neurotensin in early compartments of the regulated secretory pathway of PC5-transfected PC12 cells.  |  Barbero, P., et al. 1998. J Biol Chem. 273: 25339-46. PMID: 9738000

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neurotensin Antibody (E-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Neurotensin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neurotensin Antibody (E-5) Specifications

Alternate Product Name: Neurotensin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-170

Epitope Target: Neurotensin

Epitope Species: human

Product Reactivity/Detection: Neurotensin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Neurotensin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin