Anti-Neurotensin Antibody (E-5) recommended for detection of Neurotensin of human origin by WB, IP, IF and ELISA

Anti-Neurotensin Antibody (E-5): sc-377503

Anti-Neurotensin Antibody (E-5) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-Neurotensin Antibody (E-5) is a mouse monoclonal IgG2b κ Neurotensin antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 1-170 representing full length Neurotensin of human origin
    • Neurotensin Antibody (E-5) is recommended for detection of Neurotensin of human origin by WB, IP, IF and ELISA
    • Anti-Neurotensin Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Neurotensin (E-5): sc-377503.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for Neurotensin Antibody (E-5) for WB applications. This reagent is now offered in a bundle with Neurotensin Antibody (E-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended starting dilution for Western blot for sc-377503: Neurotensin (E-5) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-11-08
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Rated 5 out of 5 by from Produced positive Western blot data of Neurotensin Produced positive Western blot data of Neurotensin expression in non-transfected and human Neurotensin transfected NTS whole cell lysates. -SCBT QC
Date published: 2014-06-29
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