Neuroplastin CRISPR/Cas9 KO Plasmid (m): sc-422859
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neuroplastin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neuroplastin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neuroplastin CRISPR/Cas9 KO Plasmid (m) | sc-422859 | 20 µg | $397.00 |
Overview
Mouse Nptn encodes neuroplastin, a glycosylated immunoglobulin superfamily cell-adhesion protein enriched at synapses and in other membrane compartments where it supports neurite outgrowth, synaptic organization, and activity-dependent plasticity. Neuroplastin also functions as an essential auxiliary subunit of plasma membrane Ca²⁺ ATPases (PMCAs), linking Nptn to intracellular calcium homeostasis and Ca²⁺-regulated signaling that shapes excitability and gene expression. Through these roles, Nptn contributes to circuit development and synaptic remodeling processes relevant to neurodevelopmental and neuropsychiatric phenotypes, as well as broader defects arising from dysregulated calcium handling. Its membrane localization and interaction network make it a useful node for studying adhesion-dependent signaling, synapse maturation, and calcium-dependent pathways in mouse neuronal and glial models.
Neuroplastin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nptn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nptn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nptn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neuroplastin protein expression.
This CRISPR knockout system enables efficient generation of Nptn-deficient cell models for investigation of Neuroplastin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nptn exon(s) critical for Neuroplastin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nptn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neuroplastin CRISPR/Cas9 KO Plasmid (m) and Neuroplastin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nptn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neuroplastin HDR Plasmid (m) and Neuroplastin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nptn homology arms to support homology-directed repair at defined Nptn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.