Neuronatin CRISPR/Cas9 KO Plasmid (m): sc-421919
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neuronatin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neuronatin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neuronatin CRISPR/Cas9 KO Plasmid (m) | sc-421919 | 20 µg | $397.00 |
Overview
Mouse Nnat encodes neuronatin, a small imprinted, developmentally regulated protein enriched in the nervous system and endocrine tissues. Neuronatin localizes predominantly to the endoplasmic reticulum membrane and is implicated in controlling intracellular calcium dynamics, ER homeostasis, and coupling of metabolic cues to cell state decisions. Through effects on Ca2+-dependent signaling and stress-responsive pathways, neuronatin has been linked to neuronal differentiation, synaptic function, adipocyte and pancreatic β-cell biology, and regulation of energy balance. Dysregulated NNAT expression has been reported in contexts of metabolic dysfunction, neurodevelopmental phenotypes, and tumor-associated cell programs, making Nnat a useful node for mechanistic studies of development and cellular stress responses.
Neuronatin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nnat gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nnat together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nnat open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neuronatin protein expression.
This CRISPR knockout system enables efficient generation of Nnat-deficient cell models for investigation of Neuronatin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nnat exon(s) critical for Neuronatin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nnat genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neuronatin CRISPR/Cas9 KO Plasmid (m) and Neuronatin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nnat locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neuronatin HDR Plasmid (m) and Neuronatin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nnat homology arms to support homology-directed repair at defined Nnat target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.