neurexin III CRISPR/Cas9 KO Plasmid (h): sc-406719
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neurexin III CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the neurexin III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neurexin III CRISPR/Cas9 KO Plasmid (h) | sc-406719 | 20 µg | $397.00 |
Overview
NRXN3 encodes neurexin III, a presynaptic cell-adhesion molecule that organizes synapse formation and maintenance by binding postsynaptic partners such as neuroligins and other synaptic scaffolds. Through these trans-synaptic interactions, neurexin III helps regulate neurotransmitter release probability, synaptic specification, and activity-dependent remodeling that shape neuronal circuit function. NRXN3 participates in pathways governing synaptic vesicle exocytosis, calcium-dependent signaling, and excitatory/inhibitory balance across developing and mature networks. Genetic variation and altered expression of NRXN3 have been associated with neurodevelopmental and neuropsychiatric phenotypes, supporting its use in mechanistic studies of synaptic connectivity and circuit-level dysfunction.
neurexin III CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NRXN3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NRXN3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NRXN3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish neurexin III protein expression.
This CRISPR knockout system enables efficient generation of NRXN3-deficient cell models for investigation of neurexin III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NRXN3 exon(s) critical for neurexin III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NRXN3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by neurexin III CRISPR/Cas9 KO Plasmid (h) and neurexin III CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NRXN3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by neurexin III HDR Plasmid (h) and neurexin III HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NRXN3 homology arms to support homology-directed repair at defined NRXN3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.