neurexin I CRISPR/Cas9 KO Plasmid (h): sc-401872
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neurexin I CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the neurexin I genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: neurexin Iα Antibody (17): sc-136001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neurexin I CRISPR/Cas9 KO Plasmid (h) | sc-401872 | 20 µg | $397.00 |
Overview
NRXN1 encodes neurexin I, a presynaptic cell-adhesion molecule that organizes synapse formation and maturation by engaging postsynaptic partners such as neuroligins and LRRTMs. Through alternative splicing and isoform diversity, neurexin I shapes excitatory and inhibitory synaptic balance, regulates neurotransmitter release probability, and supports trans-synaptic signaling important for neuronal network connectivity. NRXN1 function intersects with pathways governing synaptic vesicle cycling, calcium-dependent exocytosis, and activity-dependent synaptic plasticity. Genetic disruption or copy number variation of NRXN1 has been linked to neurodevelopmental and neuropsychiatric phenotypes, making it a key locus for mechanistic studies in neuronal systems.
neurexin I CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NRXN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NRXN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NRXN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish neurexin I protein expression.
This CRISPR knockout system enables efficient generation of NRXN1-deficient cell models for investigation of neurexin I signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NRXN1 exon(s) critical for neurexin I function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NRXN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by neurexin I CRISPR/Cas9 KO Plasmid (h) and neurexin I CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NRXN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by neurexin I HDR Plasmid (h) and neurexin I HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NRXN1 homology arms to support homology-directed repair at defined NRXN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.