Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neu1 CRISPR/Cas9 KO Plasmid (h): sc-401488
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neu1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neu1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neu1 Antibody (F-8): sc-166824
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neu1 CRISPR/Cas9 KO Plasmid (h) | sc-401488 | 20 µg | $397.00 |
Overview
NEU1 encodes lysosomal neuraminidase 1 (Neu1), a sialidase that removes terminal sialic acids from glycoproteins and glycolipids to regulate lysosomal catabolism and cell-surface receptor turnover. Neu1 functions in concert with the protective protein/cathepsin A complex to maintain lysosomal enzyme activity and influence endocytic trafficking and autophagy-linked degradation. By modulating the sialylation state of membrane and secreted proteins, Neu1 affects immune signaling, adhesion, and extracellular matrix remodeling. Dysregulated NEU1 activity is implicated in lysosomal storage pathology and broader processes such as neuroinflammation and tumor-associated signaling networks, making it relevant for mechanistic studies of glyco-lysosomal homeostasis.
Neu1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NEU1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NEU1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NEU1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neu1 protein expression.
This CRISPR knockout system enables efficient generation of NEU1-deficient cell models for investigation of Neu1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NEU1 exon(s) critical for Neu1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NEU1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neu1 CRISPR/Cas9 KO Plasmid (h) and Neu1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NEU1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neu1 HDR Plasmid (h) and Neu1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NEU1 homology arms to support homology-directed repair at defined NEU1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.