NELF CRISPR/Cas9 KO Plasmid (m): sc-437310
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NELF CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NELF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NELF CRISPR/Cas9 KO Plasmid (m) | sc-437310 | 20 µg | $397.00 |
Overview
Nsmf encodes NELF (nasal embryonic LHRH factor), a guidance-associated protein implicated in neuronal migration and axon pathfinding during development. In mouse, NELF is linked to processes that coordinate neurite outgrowth, olfactory/vomeronasal system development, and the establishment of hypothalamic neuroendocrine circuits. Altered NSMF/NELF function has been associated with congenital hypogonadotropic hypogonadism and Kallmann syndrome–related phenotypes, reflecting roles in GnRH neuron development and reproductive axis regulation. These features make Nsmf a useful target for studying developmental neurobiology, neuroendocrine signaling, and genotype–phenotype relationships in neuronal differentiation models.
NELF CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nsmf gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nsmf together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nsmf open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NELF protein expression.
This CRISPR knockout system enables efficient generation of Nsmf-deficient cell models for investigation of NELF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nsmf exon(s) critical for NELF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nsmf genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NELF CRISPR/Cas9 KO Plasmid (m) and NELF CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nsmf locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NELF HDR Plasmid (m) and NELF HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nsmf homology arms to support homology-directed repair at defined Nsmf target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.