NEIL1 Double Nickase Plasmid (h): sc-403778-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEIL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NEIL1 Double Nickase Plasmid (h) and NEIL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NEIL1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NEIL1 Antibody (D-1): sc-271164
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEIL1 Double Nickase Plasmid (h) | sc-403778-NIC | 20 µg | $410.00 | |||
NEIL1 Double Nickase Plasmid (h2) | sc-403778-NIC-2 | 20 µg | $410.00 |
NEIL1 encodes endonuclease VIII-like 1, a DNA glycosylase/AP lyase that initiates base excision repair by recognizing and excising oxidized bases such as formamidopyrimidines and thymine glycol. NEIL1 functions in the nuclear genome and has reported roles at replication forks, supporting genome stability during S phase and coordinating repair with DNA replication and chromatin dynamics. Through interactions within BER and downstream repair synthesis, NEIL1 helps limit mutagenesis and replication stress that can arise from reactive oxygen species and endogenous DNA damage. Altered NEIL1 activity or expression has been linked in research settings to phenotypes relevant to carcinogenesis, neurodegeneration, and metabolic dysfunction, reflecting the importance of oxidative DNA damage handling in human disease biology.
NEIL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NEIL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NEIL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NEIL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NEIL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.