NEDL1 CRISPR/Cas9 KO Plasmid (h): sc-406027
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEDL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NEDL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEDL1 CRISPR/Cas9 KO Plasmid (h) | sc-406027 | 20 µg | $397.00 |
Overview
HECW1 encodes the human E3 ubiquitin-protein ligase NEDL1, a HECT-domain enzyme that catalyzes ubiquitin transfer to substrate proteins to regulate their stability and signaling output. NEDL1 participates in ubiquitin-dependent proteostasis pathways that influence protein turnover, stress responses, and cell fate decisions, and has been linked to modulation of apoptotic signaling and neuronal homeostasis. Through selective ubiquitination, NEDL1 can shape downstream transcriptional programs and quality-control networks that are frequently perturbed in neurodegeneration and cancer-associated cellular phenotypes. As a result, HECW1 is of interest for dissecting ubiquitin pathway wiring and substrate-specific regulation in human cell systems.
NEDL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HECW1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HECW1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HECW1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NEDL1 protein expression.
This CRISPR knockout system enables efficient generation of HECW1-deficient cell models for investigation of NEDL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HECW1 exon(s) critical for NEDL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HECW1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NEDL1 CRISPR/Cas9 KO Plasmid (h) and NEDL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HECW1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NEDL1 HDR Plasmid (h) and NEDL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HECW1 homology arms to support homology-directed repair at defined HECW1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.