Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NEDD4-L Double Nickase Plasmid (h): sc-403647-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEDD4-L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NEDD4-L Double Nickase Plasmid (h) and NEDD4-L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NEDD4L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NEDD4-L Antibody (C-8): sc-514954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEDD4-L Double Nickase Plasmid (h) | sc-403647-NIC | 20 µg | $410.00 | |||
NEDD4-L Double Nickase Plasmid (h2) | sc-403647-NIC-2 | 20 µg | $410.00 |
NEDD4L encodes NEDD4-L, a HECT-domain E3 ubiquitin ligase that regulates protein turnover and membrane protein abundance by catalyzing ubiquitin conjugation to specific substrates. It is a key modulator of epithelial sodium channel (ENaC) trafficking and ion homeostasis, and it also intersects with growth and stress signaling through ubiquitin-dependent control of pathway components. NEDD4-L has been implicated in regulation of TGF-β/SMAD signaling, Wnt pathway outputs, and cellular responses that shape proliferation and differentiation programs. Dysregulation of NEDD4L expression or activity is associated with altered epithelial transport physiology and has been reported in studies of cancer biology and cardiometabolic traits, supporting its use as a mechanistic node in ubiquitin signaling research.
NEDD4-L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NEDD4L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NEDD4L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NEDD4L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NEDD4L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.