Nebulette Antibody (E-12): sc-393795
- Nebulette Antibody (E-12) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 921-994 mapping near the C-terminus of Nebulette of human origin
- recommended for detection of Nebulette of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Nebulette Antibody (E-12) for WB applications. This reagent is now offered in a bundle with Nebulette Antibody (E-12) (see ordering information below).
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Nebulette Antibody (E-12) is a mouse monoclonal IgG2b light chain antibody that detects nebulette in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nebulette is a crucial protein localized specifically to the Z-disc region of the sarcomere, playing a vital role in the structural integrity and function of myofibrils in cardiac and skeletal muscle tissues. Anti-nebulette antibody (E-12) unique positioning allows nebulette to interact closely with alpha-actinin, facilitating the organization and stability of the thin filament architecture essential for muscle contraction. A polymorphism in the actin-binding motif of nebulette has been identified as a genetic marker associated with nonfamilial idiopathic dilated cardiomyopathy (IDC), a condition characterized by heart muscle thinning and impaired systolic function. Nebulette (E-12) antibody serves as an invaluable tool for researchers investigating the molecular mechanisms underlying muscle diseases and the role of nebulette in cardiac health.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nebulette Antibody (E-12) References:
- Targeted disruption of nebulette protein expression alters cardiac myofibril assembly and function. | Moncman, CL. and Wang, K. 2002. Exp Cell Res. 273: 204-18. PMID: 11822876
- Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. | Li, B., et al. 2004. J Biol Chem. 279: 20401-10. PMID: 15004028
- Expression of nebulette during early cardiac development. | Esham, M., et al. 2007. Cell Motil Cytoskeleton. 64: 258-73. PMID: 17238151
- Nebulette interacts with filamin C. | Holmes, WB. and Moncman, CL. 2008. Cell Motil Cytoskeleton. 65: 130-42. PMID: 17987659
- Ectopic expression of LIM-nebulette (LASP2) reveals roles in cell migration and spreading. | Deng, XA., et al. 2008. Cell Motil Cytoskeleton. 65: 827-40. PMID: 18677772
- Nebulette is the second member of the nebulin family fused to the MLL gene in infant leukemia. | Cóser, VM., et al. 2010. Cancer Genet Cytogenet. 198: 151-4. PMID: 20362230
- Nebulette Expression Is Associated with Lymph Node Metastasis in Patients with Colorectal Cancer. | Hosseini, SM., et al. 2018. Middle East J Dig Dis. 10: 174-179. PMID: 30186581
- Deficiency in nebulin repeats of sarcomeric nebulette is detrimental for cardiomyocyte tolerance to exercise and biomechanical stress. | Vejandla, RM., et al. 2021. Am J Physiol Heart Circ Physiol. 320: H2130-H2146. PMID: 33861145
- Nebulette: a 107 kD nebulin-like protein in cardiac muscle. | Moncman, CL. and Wang, K. 1995. Cell Motil Cytoskeleton. 32: 205-25. PMID: 8581976
- Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. | Millevoi, S., et al. 1998. J Mol Biol. 282: 111-23. PMID: 9733644
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nebulette Antibody (E-12) | sc-393795 | 200 µg/ml | $322.00 | |||
Nebulette Antibody (E-12): m-IgG2b BP-HRP Bundle | sc-548625 | 200 µg Ab; 10 µg BP | $361.00 |