NDUFS8 Antibody (A-6): sc-515527
- NDUFS8 Antibody (A-6) is a mouse monoclonal IgG1 κ NDUFS8 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 163-184 within an internal region of NDUFS8 of human origin
- NDUFS8 Antibody (A-6) is recommended for detection of NDUFS8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NDUFS8 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NDUFS8 Antibody (A-6) for WB applications. These reagents are now offered in bundles with NDUFS8 Antibody (A-6) (see ordering information below).
QUICK LINKS
NDUFS8 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDUFS8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDUFS8 (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFS8, also known as NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, plays a crucial role as a subunit of mitochondrial complex I, which is essential for the electron transport chain in oxidative phosphorylation. This complex is responsible for the oxidation of NADH generated during the Krebs cycle, facilitating the transfer of electrons to ubiquinone and ultimately contributing to ATP production and cellular energy metabolism. Proper functioning of NDUFS8 is vital, as defects in this protein can lead to severe clinical conditions, including Leigh syndrome, characterized by necrotic lesions in the brain. With its 210 amino acid structure, NDUFS8 is integral to the catalytic activity of complex I, and understanding NDUFS8 interactions and modifications is key to unraveling the complexities of mitochondrial dysfunction and associated myopathies and neuropathies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NDUFS8 Antibody (A-6) References:
- Human mitochondrial complex I in health and disease. | Smeitink, J. and van den Heuvel, L. 1999. Am J Hum Genet. 64: 1505-10. PMID: 10330338
- The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification. | Murray, J., et al. 2003. J Biol Chem. 278: 13619-22. PMID: 12611891
- Inhibition of mitochondrial Na+-Ca2+ exchange restores agonist-induced ATP production and Ca2+ handling in human complex I deficiency. | Visch, HJ., et al. 2004. J Biol Chem. 279: 40328-36. PMID: 15269216
- Clinical and molecular findings in children with complex I deficiency. | Bugiani, M., et al. 2004. Biochim Biophys Acta. 1659: 136-47. PMID: 15576045
- Assignment of the PSST subunit gene of human mitochondrial complex I to chromosome 19p13. | Hyslop, SJ., et al. 1996. Genomics. 37: 375-80. PMID: 8938450
- cDNA sequence and chromosomal localization of the NDUFS8 human gene coding for the 23 kDa subunit of the mitochondrial complex I. | Procaccio, V., et al. 1997. Biochim Biophys Acta. 1351: 37-41. PMID: 9116042
- Genomic structure of the human NDUFS8 gene coding for the iron-sulfur TYKY subunit of the mitochondrial NADH:ubiquinone oxidoreductase. | de Sury, R., et al. 1998. Gene. 215: 1-10. PMID: 9666055
- The first nuclear-encoded complex I mutation in a patient with Leigh syndrome. | Loeffen, J., et al. 1998. Am J Hum Genet. 63: 1598-608. PMID: 9837812
- The nuclear-encoded human NADH:ubiquinone oxidoreductase NDUFA8 subunit: cDNA cloning, chromosomal localization, tissue distribution, and mutation detection in complex-I-deficient patients. | Triepels, R., et al. 1998. Hum Genet. 103: 557-63. PMID: 9860297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDUFS8 Antibody (A-6) | sc-515527 | 200 µg/ml | $322.00 | |||
NDUFS8 Antibody (A-6): m-IgG Fc BP-HRP Bundle | sc-528043 | 200 µg Ab; 10 µg BP | $361.00 | |||
NDUFS8 Antibody (A-6): m-IgG1 BP-HRP Bundle | sc-533416 | 200 µg Ab; 20 µg BP | $361.00 | |||
NDUFS8 Antibody (A-6) AC | sc-515527 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NDUFS8 Antibody (A-6) HRP | sc-515527 HRP | 200 µg/ml | $322.00 | |||
NDUFS8 Antibody (A-6) FITC | sc-515527 FITC | 200 µg/ml | $336.00 | |||
NDUFS8 Antibody (A-6) PE | sc-515527 PE | 200 µg/ml | $349.00 | |||
NDUFS8 Antibody (A-6) Alexa Fluor® 488 | sc-515527 AF488 | 200 µg/ml | $364.00 | |||
NDUFS8 Antibody (A-6) Alexa Fluor® 546 | sc-515527 AF546 | 200 µg/ml | $364.00 | |||
NDUFS8 Antibody (A-6) Alexa Fluor® 594 | sc-515527 AF594 | 200 µg/ml | $364.00 | |||
NDUFS8 Antibody (A-6) Alexa Fluor® 647 | sc-515527 AF647 | 200 µg/ml | $364.00 | |||
NDUFS8 Antibody (A-6) Alexa Fluor® 680 | sc-515527 AF680 | 200 µg/ml | $364.00 | |||
NDUFS8 Antibody (A-6) Alexa Fluor® 790 | sc-515527 AF790 | 200 µg/ml | $364.00 | |||
NDUFS8 (A-6) Neutralizing Peptide | sc-515527 P | 100 µg/0.5 ml | $69.00 |