NDUFS6 Antibody (B-5): sc-518214

NDUFS6 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDUFS6 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDUFS6 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFS6, also known as NADH-ubiquinone oxidoreductase iron-sulfur protein 6, is a crucial component of complex I in the mitochondrial oxidative phosphorylation pathway, which is essential for ATP production. NDUFS6 is located in the inner mitochondrial membrane, where NDUFS6 plays a vital role in the electron transport chain by facilitating the transfer of electrons from NADH to ubiquinone, a process fundamental for cellular energy metabolism. Proper functioning of NDUFS6 is critical, as deficiencies in complex I can lead to severe metabolic disorders, including mitochondrial myopathy and neurodegenerative diseases. NDUFS6 is part of a larger multi-subunit complex, and NDUFS6 interactions with other proteins within complex I are essential for maintaining structural integrity and functional efficiency of the electron transport chain. Mutations in the NDUFS6 gene can disrupt these interactions, contributing to the pathophysiology of complex I deficiency and highlighting NDUFS6 importance in mitochondrial health and disease.

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NDUFS6 Antibody (B-5) References:

1. Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology.  |  Stojanovski, D., et al. 2004. J Cell Sci. 117: 1201-10. PMID: 14996942
2. Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q reductase gene (NDUFS1) to 2q33----q34.  |  Duncan, AM., et al. 1992. Cytogenet Cell Genet. 60: 212-3. PMID: 1505218
3. NDUFS6 mutations are a novel cause of lethal neonatal mitochondrial complex I deficiency.  |  Kirby, DM., et al. 2004. J Clin Invest. 114: 837-45. PMID: 15372108
4. Leigh syndrome associated with mitochondrial complex I deficiency due to a novel mutation in the NDUFS1 gene.  |  Martín, MA., et al. 2005. Arch Neurol. 62: 659-61. PMID: 15824269
5. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle.  |  Sparks, LM., et al. 2005. Diabetes. 54: 1926-33. PMID: 15983191
6. Ultrasound evaluation of peritoneal catheter tunnel in catheter related infections in CAPD.  |  Karahan, OI., et al. 2005. Int Urol Nephrol. 37: 363-6. PMID: 16142572
7. A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I).  |  Flemming, D., et al. 2005. J Mol Microbiol Biotechnol. 10: 208-22. PMID: 16645316
8. Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).  |  Gostimskaya, IS., et al. 2006. Biochim Biophys Acta. 1757: 1155-61. PMID: 16777054
9. Mutated NDUFS6 is the cause of fatal neonatal lactic acidemia in Caucasus Jews.  |  Spiegel, R., et al. 2009. Eur J Hum Genet. 17: 1200-3. PMID: 19259137
10. Determination of the cDNA sequence for the human mitochondrial 75-kDa Fe-S protein of NADH-coenzyme Q reductase.  |  Chow, W., et al. 1991. Eur J Biochem. 201: 547-50. PMID: 1935949
11. PRMT1 methylation of WTAP promotes multiple myeloma tumorigenesis by activating oxidative phosphorylation via m6A modification of NDUFS6.  |  Jia, Y., et al. 2023. Cell Death Dis. 14: 512. PMID: 37558663
12. Alternative splicing expands the clinical spectrum of NDUFS6-related mitochondrial disorders.  |  Armirola-Ricaurte, C., et al. 2024. Genet Med. 26: 101117. PMID: 38459834

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDUFS6 Antibody (B-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NDUFS6 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDUFS6 Antibody (B-5) Specifications

Alternate Product Name: NDUFS6

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 42-64

Epitope Target: NDUFS6

Epitope Species: human

Product Reactivity/Detection: NDUFS6

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NDUFS6 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin