NDUFS4 Antibody (A-7): sc-514002
- NDUFS4 Antibody (A-7) is a mouse monoclonal IgG1 κ NDUFS4 antibody provided at 200 µg/ml
- raised against amino acids 1-175 representing full length NDUFS4 of human origin
- NDUFS4 Antibody (A-7) is recommended for detection of NDUFS4 of human origin by WB, IP, IF and ELISA
- Anti-NDUFS4 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NDUFS4 Antibody (A-7) for WB applications. These reagents are now offered in bundles with NDUFS4 Antibody (A-7) (see ordering information below).
QUICK LINKS
NDUFS4 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDUFS4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDUFS4 (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFS4, also known as NADH dehydrogenase (ubiquinone) Fe-S protein 4, plays a crucial role as an accessory subunit of Complex I in the mitochondrial inner membrane, which is essential for cellular respiration. Complex I is responsible for transferring electrons from NADH to ubiquinone, a process that not only facilitates the generation of an electrochemical proton gradient but also drives ATP synthesis, making NDUFS4 vital for energy production in cells. Complex I′s intricate structure, composed of at least 45 different subunits, underscores the complexity of its function and the importance of each component, including NDUFS4, in maintaining mitochondrial health. Mutations in NDUFS4 can lead to severe mitochondrial respiratory chain deficiencies, resulting in a range of clinical manifestations such as cardiomyopathy, myopathy, liver failure, and neurological disorders, highlighting NDUFS4′s significance in human health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NDUFS4 Antibody (A-7) References:
- Combined enzymatic complex I and III deficiency associated with mutations in the nuclear encoded NDUFS4 gene. | Budde, SM., et al. 2000. Biochem Biophys Res Commun. 275: 63-8. PMID: 10944442
- Dysfunctions of cellular oxidative metabolism in patients with mutations in the NDUFS1 and NDUFS4 genes of complex I. | Iuso, A., et al. 2006. J Biol Chem. 281: 10374-80. PMID: 16478720
- cAMP controls oxygen metabolism in mammalian cells. | Piccoli, C., et al. 2006. FEBS Lett. 580: 4539-43. PMID: 16870178
- Mutations in structural genes of complex I associated with neurological diseases. | Scacco, S., et al. 2006. Ital J Biochem. 55: 254-62. PMID: 17274530
- Investigation of the complex I assembly chaperones B17.2L and NDUFAF1 in a cohort of CI deficient patients. | Vogel, RO., et al. 2007. Mol Genet Metab. 91: 176-82. PMID: 17383918
- cAMP-dependent protein kinase regulates the mitochondrial import of the nuclear encoded NDUFS4 subunit of complex I. | De Rasmo, D., et al. 2008. Cell Signal. 20: 989-97. PMID: 18291624
- Mice with mitochondrial complex I deficiency develop a fatal encephalomyopathy. | Kruse, SE., et al. 2008. Cell Metab. 7: 312-20. PMID: 18396137
- The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase. | Papa, S., et al. 1996. FEBS Lett. 379: 299-301. PMID: 8603710
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDUFS4 Antibody (A-7) | sc-514002 | 200 µg/ml | $322.00 | |||
NDUFS4 Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-527842 | 200 µg Ab; 10 µg BP | $361.00 | |||
NDUFS4 Antibody (A-7): m-IgG1 BP-HRP Bundle | sc-533215 | 200 µg Ab; 20 µg BP | $361.00 | |||
NDUFS4 Antibody (A-7) AC | sc-514002 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NDUFS4 Antibody (A-7) HRP | sc-514002 HRP | 200 µg/ml | $322.00 | |||
NDUFS4 Antibody (A-7) FITC | sc-514002 FITC | 200 µg/ml | $336.00 | |||
NDUFS4 Antibody (A-7) PE | sc-514002 PE | 200 µg/ml | $349.00 | |||
NDUFS4 Antibody (A-7) Alexa Fluor® 488 | sc-514002 AF488 | 200 µg/ml | $364.00 | |||
NDUFS4 Antibody (A-7) Alexa Fluor® 546 | sc-514002 AF546 | 200 µg/ml | $364.00 | |||
NDUFS4 Antibody (A-7) Alexa Fluor® 594 | sc-514002 AF594 | 200 µg/ml | $364.00 | |||
NDUFS4 Antibody (A-7) Alexa Fluor® 647 | sc-514002 AF647 | 200 µg/ml | $364.00 | |||
NDUFS4 Antibody (A-7) Alexa Fluor® 680 | sc-514002 AF680 | 200 µg/ml | $364.00 | |||
NDUFS4 Antibody (A-7) Alexa Fluor® 790 | sc-514002 AF790 | 200 µg/ml | $364.00 |