Date published: 2026-4-12

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NDUFB9 Antibody (D-7): sc-398869

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Datasheets
  • NDUFB9 Antibody (D-7) is a mouse monoclonal IgG1 κ NDUFB9 antibody, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 1-179 representing full length NDUFB9 of human origin
  • NDUFB9 Antibody (D-7) is recommended for detection of NDUFB9 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-NDUFB9 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NDUFB9 Antibody (D-7) for WB applications. These reagents are now offered in bundles with NDUFB9 Antibody (D-7) (see ordering information below).
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NDUFB9 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDUFB9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NDUFB9 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFB9, also known as NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, LYRM3, or B22, is a 179 amino acid protein that plays a crucial role as an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase complex I. This complex is essential for cellular respiration, facilitating the transfer of electrons from NADH to the respiratory chain, a process vital for ATP production and overall cellular energy metabolism. NDUFB9 is localized to the inner mitochondrial membrane and the matrix side of the peripheral membrane, which is significant because proper positioning is critical for the efficient functioning of the respiratory chain. The gene encoding NDUFB9 is located on human chromosome 8, a region associated with various diseases, including schizophrenia, bipolar disorder, and certain leukemias, highlighting NDUFB9′s importance in both normal cellular function and disease pathology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

NDUFB9 Antibody (D-7) References:

  1. Human NDUFB9 gene: genomic organization and a possible candidate gene associated with deafness disorder mapped to chromosome 8q13.  |  Lin, X., et al. 1999. Hum Hered. 49: 75-80. PMID: 10077726
  2. Human mitochondrial complex I in health and disease.  |  Smeitink, J. and van den Heuvel, L. 1999. Am J Hum Genet. 64: 1505-10. PMID: 10330338
  3. The human B22 subunit of the NADH-ubiquinone oxidoreductase maps to the region of chromosome 8 involved in branchio-oto-renal syndrome.  |  Gu, JZ., et al. 1996. Genomics. 35: 6-10. PMID: 8661098
  4. Nuclear genes of human complex I of the mitochondrial electron transport chain: state of the art.  |  Smeitink, JA., et al. 1998. Hum Mol Genet. 7: 1573-9. PMID: 9735378
  5. Intron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain.  |  Emahazion, T., et al. 1998. Cytogenet Cell Genet. 82: 115-9. PMID: 9763677
  6. cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.  |  Loeffen, JL., et al. 1998. Biochem Biophys Res Commun. 253: 415-22. PMID: 9878551

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

NDUFB9 Antibody (D-7)

sc-398869
200 µg/ml
$322.00

NDUFB9 Antibody (D-7): m-IgG Fc BP-HRP Bundle

sc-527827
200 µg Ab; 10 µg BP
$361.00

NDUFB9 Antibody (D-7): m-IgG1 BP-HRP Bundle

sc-533200
200 µg Ab; 20 µg BP
$361.00

NDUFB9 Antibody (D-7) AC

sc-398869 AC
500 µg/ml, 25% agarose
$424.00

NDUFB9 Antibody (D-7) HRP

sc-398869 HRP
200 µg/ml
$322.00

NDUFB9 Antibody (D-7) FITC

sc-398869 FITC
200 µg/ml
$336.00

NDUFB9 Antibody (D-7) PE

sc-398869 PE
200 µg/ml
$349.00

NDUFB9 Antibody (D-7) Alexa Fluor® 488

sc-398869 AF488
200 µg/ml
$364.00

NDUFB9 Antibody (D-7) Alexa Fluor® 546

sc-398869 AF546
200 µg/ml
$364.00

NDUFB9 Antibody (D-7) Alexa Fluor® 594

sc-398869 AF594
200 µg/ml
$364.00

NDUFB9 Antibody (D-7) Alexa Fluor® 647

sc-398869 AF647
200 µg/ml
$364.00

NDUFB9 Antibody (D-7) Alexa Fluor® 680

sc-398869 AF680
200 µg/ml
$364.00

NDUFB9 Antibody (D-7) Alexa Fluor® 790

sc-398869 AF790
200 µg/ml
$364.00

What is the recommended starting dilution for Western blot for sc-398869: NDUFB9 (D-7) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
  • y_2026, m_4, d_9, h_7CST
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Rated 5 out of 5 by from Works well.I've tested this antibody with HeLa and 143B lysates and found a clean band at ~21KDa.
Date published: 2017-11-08
Rated 5 out of 5 by from very clean, specific bandGave a great signal at the correct molecular weight with no background non-specific bands.
Date published: 2017-10-23
Rated 5 out of 5 by from WB publishable data with human colorectalWB publishable data with human colorectal cancer lines - SCBT Publication Review
Date published: 2013-04-06
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NDUFB9 Antibody (D-7) is rated 5.0 out of 5 by 3.
  • y_2026, m_4, d_9, h_7
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