NDST Antibody (E-9): sc-374529

NDST Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDST protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NDST (E-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDST protein plays a crucial role in the modification of heparan sulfate and heparin, specifically through the N-deacetylation and N-sulfation of N-acetylglucosamine residues. This modification is essential for the synthesis of oligosaccharide sequences that possess specific ligand-binding properties, which are vital for various biological processes, including cell signaling, growth factor activity, and the regulation of cell adhesion. NDSTs′ enzymatic activity is mediated by monomeric enzymes that exhibit two catalytic functions, and multiple isozymes of NDST have been identified, each with distinct tissue distributions and enzymatic characteristics. Phylogenetic studies indicate that the NDST family, comprising NDST1-4, evolved from a common ancestral gene, leading to the emergence of two subtypes, NDST1/2 and NDST3/4. NDST1, located on human chromosome 5q32-q33.1, shares significant homology with NDST2, which is found on chromosome 10q22, while NDST3 and NDST4 are closely linked on chromosome 4, mapping to 4q25-26 and 4q26-27, respectively. Expression patterns of NDST3 and NDST4 mRNA in various mouse tissues are more restricted compared to the abundant and ubiquitous expression of NDST1 and NDST2, highlighting the functional diversity and regulatory mechanisms of these enzymes in different biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NDST Antibody (E-9) References:

1. Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4.  |  Aikawa, J., et al. 2001. J Biol Chem. 276: 5876-82. PMID: 11087757
2. Cloning of the human heparan sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher Collins syndrome candidate region at 5q32-q33.1.  |  Dixon, J., et al. 1995. Genomics. 26: 239-44. PMID: 7601448
3. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2.  |  Humphries, DE., et al. 1998. Biochem J. 332 (Pt 2): 303-7. PMID: 9601056
4. Molecular cloning and expression of a third member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family.  |  Aikawa, J. and Esko, JD. 1999. J Biol Chem. 274: 2690-5. PMID: 9915799

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDST Antibody (E-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-NDST Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDST Antibody (E-9) Specifications

Alternate Product Name: NDST

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 583-882

Epitope Target: NDST

Epitope Species: human

Product Reactivity/Detection: NDST1, NDST2, NDST3 and NDST4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for NDST Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin