Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NDRG3 Double Nickase Plasmid (m): sc-424349-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDRG3 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NDRG3 Double Nickase Plasmid (m) and NDRG3 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ndrg3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDRG3 Antibody (H-11): sc-514561
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDRG3 Double Nickase Plasmid (m) | sc-424349-NIC | 20 µg | $410.00 | |||
NDRG3 Double Nickase Plasmid (m2) | sc-424349-NIC-2 | 20 µg | $410.00 |
Mouse Ndrg3 encodes NDRG3, a member of the NDRG family implicated in cellular stress adaptation, metabolic regulation, and differentiation programs. NDRG3 has been linked to hypoxia-associated signaling and lactate-responsive pathways that can influence cell survival, proliferation, and transcriptional outputs. In many contexts, NDRG3 activity intersects with processes controlling mitochondrial metabolism, redox balance, and developmental gene expression. Dysregulation of NDRG3-related networks has been associated in the literature with altered growth control and disease-relevant phenotypes, supporting its use as a functional node in pathway and mechanism studies.
NDRG3 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ndrg3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ndrg3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ndrg3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ndrg3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.