Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NDR1 CRISPR/Cas9 KO Plasmid (h): sc-402716
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NDR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDR1 Antibody (A-8): sc-365555
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDR1 CRISPR/Cas9 KO Plasmid (h) | sc-402716 | 20 µg | $397.00 |
Overview
STK38 encodes the serine/threonine kinase NDR1, an AGC family kinase that functions downstream of MST1/2 in Hippo-related signaling to regulate cell-cycle progression, centrosome biology, apoptosis, and cytoskeletal organization. NDR1 activity is controlled by phosphorylation and cofactor binding (including MOB proteins), linking upstream stress and polarity cues to transcriptional and structural outputs. Through roles in mitotic control, DNA damage–associated responses, and epithelial homeostasis, STK38/NDR1 has been studied in contexts relevant to tumor biology, neurobiology, and inflammation-related signaling. Perturbation of NDR1-dependent pathways can influence proliferation, survival, and migration phenotypes that are commonly interrogated in mechanistic disease models.
NDR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STK38 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STK38 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STK38 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NDR1 protein expression.
This CRISPR knockout system enables efficient generation of STK38-deficient cell models for investigation of NDR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STK38 exon(s) critical for NDR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STK38 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NDR1 CRISPR/Cas9 KO Plasmid (h) and NDR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STK38 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NDR1 HDR Plasmid (h) and NDR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STK38 homology arms to support homology-directed repair at defined STK38 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.