Ndfip1 Antibody (NiNterm20-5): sc-53956

Ndfip1 Antibody (NiNterm20-5) is a hamster monoclonal IgG2b antibody that detects Nedd4 family interacting protein-1 (Ndfip1) in mouse samples through applications such as western blotting (WB) and flow cytometry (FCM). Ndfip1 is a 221 amino acid protein that plays a crucial role in the Golgi apparatus, where Ndfip1 is associated with maintaining Golgi structure and function. Ndfip1 features three transmembrane domains at its carboxy terminus and two proline-tyrosine (PY) motifs at its amino terminus, which are essential for interaction with WW domains of various Nedd4 family members, specifically HECT-type E3 ubiquitin ligases. Ndfip1′s significance extends to neuroprotective properties, as Ndfip1 is strongly expressed in surviving neurons near injury sites, suggesting a survival strategy through ubiquitination. Furthermore, Ndfip1′s interaction with the E3 ubiquitin ligase Itch is particularly noteworthy, as Ndfip1 expression can be enhanced by T cell activation, indicating a potential role in immune responses. Mice deficient in Ndfip1 demonstrate severe skin and lung inflammation due to Itch inactivation and subsequent JunB accumulation, leading to premature death. Anti-Ndfip1 antibody (NiNterm20-5) serves as an invaluable tool for researchers investigating Ndfip1′s intricate roles in cellular processes and implications in neuroprotection and immune regulation.

The preferred Ndfip1 antibody is Ndfip1 Antibody (D-4): sc-398469.