NCK1/2 Antibody (G-12): sc-166425

NCK1/2 Antibody (G-12) is a mouse monoclonal IgG1 antibody that detects NCK1/2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NCK1/2 proteins play a crucial role in cellular signaling by acting as adaptor proteins that link tyrosine kinase receptors, such as the epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor (PDGFR), to downstream signaling pathways. The importance of NCK1/2 lies in their ability to mediate the interaction between various signaling molecules, which is essential for processes like cell proliferation, migration, and cytoskeletal organization. Specifically, the SH3 domain of NCK binds to proline-rich regions on proteins like PAK, a key regulator of the actin cytoskeleton, thereby facilitating the coordination of actin dynamics in response to growth factor stimulation. Additionally, NCK2 has been shown to bind to the phosphorylated form of PDGFR-β, inhibiting PDGF-induced cellular responses such as membrane ruffling and lamellipod formation, which are critical for cell movement and tissue remodeling. The human NCK1 and NCK2 genes are located on chromosomes 3q21 and 2q12, respectively, highlighting their evolutionary significance and potential implications in various diseases, including cancer. NCK1/2 (G-12) antibody is an invaluable tool for researchers studying cellular signaling pathways and their implications in health and disease.

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NCK1/2 Antibody (G-12) References:

1. The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.  |  Frese, S., et al. 2006. J Biol Chem. 281: 18236-45. PMID: 16636066
2. OSU-03012 sensitizes breast cancers to lapatinib-induced cell killing: a role for Nck1 but not Nck2.  |  West, NW., et al. 2013. BMC Cancer. 13: 256. PMID: 23706161
3. Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics.  |  Buvall, L., et al. 2013. Nat Commun. 4: 2863. PMID: 24287595
4. Non-overlapping functions of Nck1 and Nck2 adaptor proteins in T cell activation.  |  Ngoenkam, J., et al. 2014. Cell Commun Signal. 12: 21. PMID: 24670066
5. Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis.  |  Jacquet, K., et al. 2018. Mol Cell Proteomics. 17: 1979-1990. PMID: 30002203
6. Aristolochic acid inhibits Slit2-induced migration and tube formation via inactivation of Robo1/Robo2-NCK1/NCK2 signaling pathway in human umbilical vein endothelial cells.  |  Guan, T., et al. 2019. Toxicol Lett. 300: 51-58. PMID: 30381256
7. Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability.  |  Alfaidi, M., et al. 2020. J Am Heart Assoc. 9: e016099. PMID: 32468886
8. Nck adaptors at a glance.  |  Bywaters, BC. and Rivera, GM. 2021. J Cell Sci. 134: PMID: 34558601
9. Endothelial NCK2 promotes atherosclerosis progression in male but not female Nck1-null atheroprone mice.  |  Bywaters, BC., et al. 2022. Front Cardiovasc Med. 9: 955027. PMID: 36035930
10. Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development.  |  Golding, AP., et al. 2023. J Mammary Gland Biol Neoplasia. 28: 19. PMID: 37479911

The preferred NCK2 antibody is NCK2 Antibody (8.8): sc-20020.