Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NCAM-L1 Double Nickase Plasmid (h): sc-401012-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NCAM-L1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NCAM-L1 Double Nickase Plasmid (h) and NCAM-L1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting L1CAM. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NCAM-L1 Antibody (D-5): sc-374046
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NCAM-L1 Double Nickase Plasmid (h) | sc-401012-NIC | 20 µg | $410.00 | |||
NCAM-L1 Double Nickase Plasmid (h2) | sc-401012-NIC-2 | 20 µg | $410.00 |
Human L1CAM encodes the neural cell adhesion molecule L1 (NCAM-L1), a transmembrane Ig superfamily protein that mediates homophilic and heterophilic adhesion to coordinate neurite outgrowth, axon guidance, and neuronal migration. NCAM-L1 participates in cytoskeletal remodeling and signal transduction through pathways involving ankyrin linkage, MAPK/ERK and PI3K signaling, and regulation of growth cone dynamics. Outside the nervous system, L1CAM supports cell–cell interactions and motility programs that influence tissue organization. Dysregulated L1CAM expression or mutation is associated with neurodevelopmental disorders and has been studied as a marker of invasive behavior in several cancers, linking adhesion signaling to disease-relevant phenotypes.
NCAM-L1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the L1CAM locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within L1CAM. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt L1CAM function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of L1CAM-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.