Na+ CP type Iα CRISPR/Cas9 KO Plasmid (m): sc-422818
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Na+ CP type Iα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Na+ CP type Iα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Na+ CP type Iα CRISPR/Cas9 KO Plasmid (m) | sc-422818 | 20 µg | $397.00 |
Overview
Scn1a encodes the voltage-gated sodium channel α subunit Na+ CP type Iα, a principal determinant of action potential initiation and propagation in neurons. By governing fast inward Na+ currents at the axon initial segment and nodes of Ranvier, it shapes neuronal firing thresholds, spike timing, and network synchrony. Scn1a function intersects with excitatory–inhibitory balance, activity-dependent signaling, and ion homeostasis pathways that influence synaptic transmission and circuit stability. Genetic and functional perturbation of SCN1A is strongly linked to seizure susceptibility and neurodevelopmental phenotypes, making it a key locus for mechanistic studies in murine models.
Na+ CP type Iα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scn1a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scn1a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scn1a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Na+ CP type Iα protein expression.
This CRISPR knockout system enables efficient generation of Scn1a-deficient cell models for investigation of Na+ CP type Iα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scn1a exon(s) critical for Na+ CP type Iα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scn1a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Na+ CP type Iα CRISPR/Cas9 KO Plasmid (m) and Na+ CP type Iα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scn1a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Na+ CP type Iα HDR Plasmid (m) and Na+ CP type Iα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scn1a homology arms to support homology-directed repair at defined Scn1a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.