NAP1L3 CRISPR/Cas9 KO Plasmid (h): sc-407403
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NAP1L3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NAP1L3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NAP1L3 CRISPR/Cas9 KO Plasmid (h) | sc-407403 | 20 µg | $397.00 |
Overview
NAP1L3 (nucleosome assembly protein 1-like 3) is a histone chaperone of the NAP1 family implicated in nucleosome assembly and disassembly, supporting chromatin dynamics during DNA replication, transcription, and DNA repair. By modulating histone H2A–H2B deposition and exchange, NAP1L3 can influence epigenetic regulation, genome stability, and cell-state transitions linked to differentiation programs. Altered chromatin remodeling and histone chaperone activity are frequently associated with dysregulated transcriptional networks observed in cancer and developmental disorders, making NAP1L3 a useful node for mechanistic studies. Investigating NAP1L3 function can help define how chromatin assembly pathways interface with replication stress responses and transcriptional control.
NAP1L3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NAP1L3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NAP1L3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NAP1L3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NAP1L3 protein expression.
This CRISPR knockout system enables efficient generation of NAP1L3-deficient cell models for investigation of NAP1L3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NAP1L3 exon(s) critical for NAP1L3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NAP1L3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NAP1L3 CRISPR/Cas9 KO Plasmid (h) and NAP1L3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NAP1L3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NAP1L3 HDR Plasmid (h) and NAP1L3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NAP1L3 homology arms to support homology-directed repair at defined NAP1L3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.