Nap1 Antibody (D-2): sc-25342

Nap1 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nap1 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Nap1 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nap1 protein plays a crucial role in cell cycle regulation, particularly in G1 to S phase transition, where Nap1 interacts with cyclin-dependent protein kinase Cdc28 and G1 cyclins Cln1, Cln2, and Cln3. Such interaction ensures cells only proceed to DNA synthesis when adequately prepared. Nap1 is also essential for Clb2 function, a cyclin involved in G2 to M phase transition, facilitating specific mitotic events and transition from polar to isotropic bud growth. Nap1′s ability to modulate these critical processes underscores Nap1′s importance in maintaining cellular integrity and function, making anti-Nap1 antibody (D-2) an invaluable tool for researchers studying cell cycle dynamics in yeast.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nap1 Antibody (D-2) References:

1. Members of the NAP/SET family of proteins interact specifically with B-type cyclins.  |  Kellogg, DR., et al. 1995. J Cell Biol. 130: 661-73. PMID: 7622566
2. NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast.  |  Kellogg, DR. and Murray, AW. 1995. J Cell Biol. 130: 675-85. PMID: 7622567
3. Starting to cycle: G1 controls regulating cell division in budding yeast.  |  Sherlock, G. and Rosamond, J. 1993. J Gen Microbiol. 139: 2531-41. PMID: 8277239
4. Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins.  |  Amon, A., et al. 1993. Cell. 74: 993-1007. PMID: 8402888
5. Control of the yeast cell cycle by the Cdc28 protein kinase.  |  Nasmyth, K. 1993. Curr Opin Cell Biol. 5: 166-79. PMID: 8507488
6. G2 cyclins are required for the degradation of G1 cyclins in yeast.  |  Blondel, M. and Mann, C. 1996. Nature. 384: 279-82. PMID: 8918881
7. Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities.  |  Levine, K., et al. 1996. Mol Cell Biol. 16: 6794-803. PMID: 8943334
8. Control of mitotic events by Nap1 and the Gin4 kinase.  |  Altman, R. and Kellogg, D. 1997. J Cell Biol. 138: 119-30. PMID: 9214386

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nap1 Antibody (D-2) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nap1 Antibody (D-2) Specifications

Alternate Product Name: Nap1

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-417

Epitope Target: Nap1

Epitope Species: S. cerevisiae

Product Reactivity/Detection: Nap1

Species Reactivity/Detection: y

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Nap1 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin