Nanos2 CRISPR/Cas9 KO Plasmid (h): sc-404123
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nanos2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nanos2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nanos2 Antibody (B-12): sc-393794
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nanos2 CRISPR/Cas9 KO Plasmid (h) | sc-404123 | 20 µg | $397.00 |
Overview
NANOS2 encodes Nanos2, an evolutionarily conserved RNA-binding protein that acts as a post-transcriptional regulator of germ cell fate. In human biology, NANOS2 is linked to maintenance of male germline identity by promoting spermatogonial differentiation programs while repressing aberrant entry into meiosis through control of mRNA stability and translation. Its function intersects with germline regulatory networks and RNA granule–associated processes that shape transcriptome remodeling during gametogenesis. Dysregulation of germ cell developmental pathways involving NANOS family proteins is relevant to studies of infertility, gonadal development disorders, and germ cell tumor biology.
Nanos2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NANOS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NANOS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NANOS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nanos2 protein expression.
This CRISPR knockout system enables efficient generation of NANOS2-deficient cell models for investigation of Nanos2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NANOS2 exon(s) critical for Nanos2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NANOS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nanos2 CRISPR/Cas9 KO Plasmid (h) and Nanos2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NANOS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nanos2 HDR Plasmid (h) and Nanos2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NANOS2 homology arms to support homology-directed repair at defined NANOS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.