Nanos2 Antibody (B-12): sc-393794
- Nanos2 Antibody (B-12) is a mouse monoclonal IgG2a κ Nanos2 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 38-67 within an internal region of Nanos2 of human origin
- Nanos2 Antibody (B-12) is recommended for detection of Nanos2 of human origin by WB, IP, IF and ELISA
- Anti-Nanos2 Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Nanos2 Antibody (B-12) for WB applications. These reagents are now offered in bundles with Nanos2 Antibody (B-12) (see ordering information below).
QUICK LINKS
Nanos2 Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nanos2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nanos2 (B-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nanos2, also known as NOS2, is a 138 amino acid protein characterized by a nanos-type zinc finger structure, which consists of two C2HC motifs that bind zinc ions. This structural feature is crucial as Nanos2 facilitates translational regulation, particularly in male germ cells where Nanos2 is specifically expressed. Nanos2 is essential for spermatogonia formation and supports self-renewal, proliferation, and overall development of proximal germ cells. Nanos2 regulates translation of target mRNAs by potentially interacting with the 3′-untranslated region of specific transcripts, thereby influencing gene expression during critical stages of germ cell development. The gene encoding human Nanos2 is located on chromosome 19, a region that also harbors several immunoglobulin superfamily members, including killer cell and leukocyte immunoglobulin-like receptors, various intercellular adhesion molecules, and Fc receptors, highlighting Nanos2′s significance in the broader context of immune response and cellular communication.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nanos2 Antibody (B-12) References:
- Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. | Jaruzelska, J., et al. 2003. Dev Genes Evol. 213: 120-6. PMID: 12690449
- Conserved role of nanos proteins in germ cell development. | Tsuda, M., et al. 2003. Science. 301: 1239-41. PMID: 12947200
- Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline. | D'Agostino, I., et al. 2006. Dev Biol. 292: 244-52. PMID: 16499902
- Implication of nanos2-3'UTR in the expression and function of nanos2. | Tsuda, M., et al. 2006. Mech Dev. 123: 440-9. PMID: 16806845
- Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell development. | Suzuki, A., et al. 2007. Development. 134: 77-83. PMID: 17138666
- Nanos2 suppresses meiosis and promotes male germ cell differentiation. | Suzuki, A. and Saga, Y. 2008. Genes Dev. 22: 430-5. PMID: 18281459
- Sexual development of mouse germ cells: Nanos2 promotes the male germ cell fate by suppressing the female pathway. | Saga, Y. 2008. Dev Growth Differ. 50 Suppl 1: S141-7. PMID: 18430166
- Mouse germ cell development during embryogenesis. | Saga, Y. 2008. Curr Opin Genet Dev. 18: 337-41. PMID: 18625315
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nanos2 Antibody (B-12) | sc-393794 | 200 µg/ml | $322.00 | |||
Nanos2 Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-526269 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nanos2 Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-546551 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nanos2 Antibody (B-12) AC | sc-393794 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nanos2 Antibody (B-12) HRP | sc-393794 HRP | 200 µg/ml | $322.00 | |||
Nanos2 Antibody (B-12) FITC | sc-393794 FITC | 200 µg/ml | $336.00 | |||
Nanos2 Antibody (B-12) PE | sc-393794 PE | 200 µg/ml | $349.00 | |||
Nanos2 Antibody (B-12) Alexa Fluor® 488 | sc-393794 AF488 | 200 µg/ml | $364.00 | |||
Nanos2 Antibody (B-12) Alexa Fluor® 546 | sc-393794 AF546 | 200 µg/ml | $364.00 | |||
Nanos2 Antibody (B-12) Alexa Fluor® 594 | sc-393794 AF594 | 200 µg/ml | $364.00 | |||
Nanos2 Antibody (B-12) Alexa Fluor® 647 | sc-393794 AF647 | 200 µg/ml | $364.00 | |||
Nanos2 Antibody (B-12) Alexa Fluor® 680 | sc-393794 AF680 | 200 µg/ml | $364.00 | |||
Nanos2 Antibody (B-12) Alexa Fluor® 790 | sc-393794 AF790 | 200 µg/ml | $364.00 | |||
Nanos2 (B-12) Neutralizing Peptide | sc-393794 P | 100 µg/0.5 ml | $69.00 |