Nanos1 Antibody (5F12): sc-293352
- Nanos1 Antibody (5F12) is a mouse monoclonal IgG1 κ Nanos1 antibody provided at 100 µg/ml
- raised against amino acids 206-270 of Nanos1 of human origin
- recommended for detection of Nanos1 of mouse, rat and human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nanos1 Antibody (5F12). This work is in progress.
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Nanos1 Antibody (5F12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nanos1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Nanos1 antibody (5F12) is available as the non-conjugated form. Nanos1, also known as NOS1, is a crucial 292 amino acid protein that plays a significant role in the regulation of germline stem cells, primarily by localizing to the perinuclear region of the cytoplasm and containing a nanos-type zinc finger. Nanos1 is highly expressed in spermatogonia and is present at lower levels in fetal ovaries, where Nanos1 forms a complex with Pumilio 2 to regulate the translation of specific mRNAs through interaction with the 3′-untranslated region of target mRNAs. Nanos1 plays an essential function in preventing premature entry into oogenesis, thereby ensuring the proper establishment and maintenance of germline stem cells. The gene encoding Nanos1 is located on human chromosome 10, a region that contains over 1,200 genes and is implicated in various genetic disorders, including Charcot-Marie-Tooth disease and multiple endocrine neoplasia type 2. With a critical role in stem cell biology and potential implications in developmental disorders, anti-Nanos1 antibody (5F12) serves as a valuable tool for researchers studying germline development and related pathways.
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Nanos1 Antibody (5F12) References:
- Proteomic analysis of NMDA receptor-adhesion protein signaling complexes. | Husi, H., et al. 2000. Nat Neurosci. 3: 661-9. PMID: 10862698
- Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. | Jaruzelska, J., et al. 2003. Dev Genes Evol. 213: 120-6. PMID: 12690449
- Nanos maintains germline stem cell self-renewal by preventing differentiation. | Wang, Z. and Lin, H. 2004. Science. 303: 2016-9. PMID: 14976263
- E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion. | Strumane, K., et al. 2006. Cancer Res. 66: 10007-15. PMID: 17047063
- The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. | Bonnomet, A., et al. 2008. Oncogene. 27: 3692-9. PMID: 18223680
- The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells. | Ginter-Matuszewska, B., et al. 2009. Mol Hum Reprod. 15: 173-9. PMID: 19168546
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nanos1 Antibody (5F12) | sc-293352 | 100 µg/ml | $316.00 |