Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NALP1 Double Nickase Plasmid (h): sc-402588-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NALP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NALP1 Double Nickase Plasmid (h) and NALP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NLRP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NALP1 Antibody (B-2): sc-166368
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NALP1 Double Nickase Plasmid (h) | sc-402588-NIC | 20 µg | $410.00 | |||
NALP1 Double Nickase Plasmid (h2) | sc-402588-NIC-2 | 20 µg | $410.00 |
Human NLRP1 encodes the sensor protein NALP1, a nucleotide-binding leucine-rich repeat receptor that nucleates inflammasome assembly in response to cellular stress and pathogen-associated signals. Upon activation, NALP1 promotes caspase-1 activation and maturation of IL‑1β and IL‑18, linking innate immune detection to pyroptotic signaling and inflammatory cytokine release. This pathway intersects with NF‑κB priming, proteostasis surveillance, and cell death checkpoints that shape epithelial and immune homeostasis. Dysregulated NLRP1 activity has been associated with inflammatory and autoimmune phenotypes and is frequently studied in the context of barrier-tissue inflammation and stress-induced innate immune signaling.
NALP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NLRP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NLRP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NLRP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NLRP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.