alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (9-A5): sc-58629

Na+/K+-ATPase α1 Antibody (9-A5) is a mouse monoclonal IgG1 kappa light chain antibody, specifically designed to recognize the Na+/K+-ATPase α1 subunit, also known as ATP1A1 or the sodium-potassium pump. Na+/K+-ATPase α1 monoclonal antibody (9-A5) was generated against the rat Na+/K+-ATPase α1 protein and exhibits reactivity with the α1 subunits from mouse, rat, human, and canine origins, making it versatile for various research applications including western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). The Na+/K+-ATPase is a vital membrane-bound enzyme that maintains essential electrochemical gradients by actively transporting three sodium ions out of the cell and two potassium ions into the cell, a process crucial for cellular volume regulation, resting membrane potential, and secondary active transport of other solutes. Structurally, the α1 subunit serves as the catalytic core of the enzyme, containing the binding sites for ATP and the transported ions, while assembling with the β and γ subunits to form a stable oligomeric complex. The β subunit is glycosylated, ensuring proper folding and membrane insertion of the α1 subunit, whereas the γ subunit modulates the enzyme′s affinity for ATP, particularly in specialized tissues such as nephron segments. Understanding the detailed structure of Na+/K+-ATPase is essential, as this knowledge elucidates the mechanism by which the enzyme facilitates ion transport and regulation within various cellular environments. Na+/K+-ATPase α1 (9-A5) antibody is an indispensable tool for researchers investigating ion transport dynamics, cellular homeostasis, and the molecular basis of related physiological processes, providing reliable and consistent detection of the Na+/K+-ATPase α1 subunit across multiple species and experimental settings.

The preferred alpha 1 Sodium Potassium ATPase/ATP1A1 antibody is alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6): sc-21712.