beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8): sc-21713

Na+/K+-ATPase β1 Antibody (C464.8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Na+/K+-ATPase beta 1 protein of mouse, rat, human, rabbit, and canine origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Na+/K+-ATPase β1 (C464.8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Na+/K+-ATPase beta 1 protein plays a crucial role in the proper functioning of the Na+/K+-ATPase complex, which is essential for maintaining cellular homeostasis. Na+/K+-ATPase beta 1 is primarily located in the plasma membrane of cells, where Na+/K+-ATPase beta 1 is involved in the regulation of ion gradients by facilitating the active transport of sodium and potassium ions across the membrane. Correct localization of Na+/K+-ATPase beta 1 ensures efficient functioning of the Na+/K+-ATPase pump, which is critical for processes such as maintaining the resting membrane potential, regulating cell volume, and supporting various cellular signaling pathways. Interaction between the beta subunit and alpha subunit of the Na+/K+-ATPase complex is particularly important, as the beta subunit aids in proper folding and insertion of the alpha subunit into the membrane while influencing pump activity. Anti-Na+/K+-ATPase β1 antibody (C464.8) serves as a valuable tool for researchers studying physiological and pathological roles of this essential protein in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) References:

1. Functional role and immunocytochemical localization of the gamma a and gamma b forms of the Na,K-ATPase gamma subunit.  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
2. Chemical modification as an approach to elucidation of sodium pump structure-function relations.  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
3. The sodium pump needs its beta subunit.  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
4. Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
5. A proteolipid associated with Na,K-ATPase is not essential for ATPase activity.  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
6. Functional significance of the beta-subunit for heterodimeric P-type ATPases.  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
7. Na+,K(+)-ATPase.  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
8. Subunit requirements for expression of functional sodium pumps in yeast cells.  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
9. The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit.  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
10. Expression, targeting, and assembly of functional Na,K-ATPase polypeptides in baculovirus-infected insect cells.  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
11. Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
12. Structure and function of the Na,K pump: ten years of molecular biology.  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) Specifications

Alternate Product Name: ATP1B1

Product Clone Name: C464.8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Na+/K+-ATPase β1

Epitope Species: rabbit

Product Reactivity/Detection: Na+/K+-ATPase β1

Species Reactivity/Detection: mouse, rat, human, rabbit, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin