alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2): sc-514614

Na+/K+-ATPase α1 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody designed to specifically recognize the Na+/K+-ATPase α1 subunit, also known as the Sodium-Potassium pump, of human origin. Na+/K+-ATPase α1 monoclonal antibody (F-2) targets an epitope mapping between amino acids 54-76 near the N-terminus, ensuring accurate detection of the protein in various applications. Na+/K+-ATPase α1 is a crucial membrane-bound enzyme located in the plasma membrane, responsible for maintaining the electrochemical gradients of sodium and potassium ions by actively transporting three Na⁺ ions out of the cell and two K⁺ ions into the cell using ATP hydrolysis. This protein undergoes important post-translational modifications, including glycosylation, which are essential for proper folding, stability, membrane localization, and functional activity. Glycosylation of the β subunit is particularly important for the correct assembly and insertion of the α1 subunit into the plasma membrane, while phosphorylation events regulate activity and interactions with other cellular proteins. Na+/K+-ATPase α1 (F-2) antibody is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), demonstrating broad reactivity with Na+/K+-ATPase α1 from mouse, rat, human, and monkey, as well as additional species including ovine, porcine, and canine. This extensive species reactivity makes Na+/K+-ATPase α1 (F-2) monoclonal antibody an invaluable tool for researchers studying ion transport mechanisms, cellular homeostasis, and related pathophysiological conditions. Understanding the post-translational modifications of Na+/K+-ATPase α1 is crucial for elucidating its role in maintaining cellular functions and involvement in diseases such as hypertension, cardiac dysfunction, and neurological disorders. Na+/K+-ATPase α1 (F-2) antibody is an essential reagent for advancing scientific research into the complex regulation and function of this pivotal membrane protein.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) Specifications

Alternate Product Name: ATP1A1

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 54-76

Epitope Target: Na⁺/K⁺-ATPase α1

Epitope Species: human

Product Reactivity/Detection: Na⁺/K⁺-ATPase α1

Species Reactivity/Detection: mouse, rat, human, monkey

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred alpha 1 Sodium Potassium ATPase/ATP1A1 antibody is alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6): sc-21712.