Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) recommended for detection of Na+/K+-ATPase α1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA

Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2): sc-514614

Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) is rated 4.0 out of 5 by 5.
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Datasheets

    • Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) is a mouse monoclonal IgG1 κ alpha 1 Sodium Potassium ATPase/ATP1A1 antibody, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 54-76 near the N-terminus of Na+/K+-ATPase α1 of human origin
    • Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) is recommended for detection of Na+/K+-ATPase α1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of alpha 1 Sodium Potassium ATPase/ATP1A1 (F-2): sc-514614.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) for WB and IHC(P) applications. These reagents are now offered in bundles with alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (F-2) (see ordering information below).
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What application is the blocking peptide sc-514614 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good Antibody Used with 40 ug of protein extract from bronchial epithelial cells and worked well, it needs long exposure but the result is very clean and specific.
Date published: 2021-04-22
Rated 5 out of 5 by from Works Great by immunofluorescence. Worked at 1:500 on rat brain tissue formalin fixed and paraffin embedded. Great product.
Date published: 2020-11-30
Rated 5 out of 5 by from Very works with western blotting This antibody very works with western blotting with SAS cell lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from This antibody works great Very specific neuronal membrane stain. IgG3 so you can combine with many other antibodies!
Date published: 2019-01-24
Rated 1 out of 5 by from Didn't worked for western blotting Didn't worked for western blotting when used for detecting membrane proteins from MCF-7, Jurkat and PC-3 cells
Date published: 2019-01-02
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