alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6): sc-21712

Na+/K+-ATPase α1 Antibody (C464.6) is a mouse monoclonal IgG1 kappa light chain antibody engineered to recognize Na+/K+-ATPase α1 (ATP1A1) across multiple species in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Na+/K+-ATPase α1 monoclonal antibody (C464.6) is available in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, supporting diverse research needs. Na+/K+-ATPase is a crucial membrane protein in the P-type ion transporter family, maintaining cellular homeostasis by actively pumping three sodium ions out and two potassium ions into cells against electrochemical gradients. This action regulates cellular volume, maintains resting membrane potential, and enables secondary active transport of various solutes. Na+/K+-ATPase complex comprises multiple isoforms of α, β, and γ subunits; α1 subunit contains ATP and ion binding sites, while β subunit ensures proper folding and membrane insertion of α subunits. γ subunit enhances ATP affinity in specific tissues, particularly nephron segments in kidneys. Na+/K+-ATPase α1 (C464.6) antibody enables researchers to investigate this essential protein′s regulation and role in various biological contexts, advancing understanding of cellular mechanisms, hypertension, cardiac function, and neurological disorders.

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alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6) References:

1. Functional role and immunocytochemical localization of the gamma a and gamma b forms of the Na,K-ATPase gamma subunit.  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
2. Chemical modification as an approach to elucidation of sodium pump structure-function relations.  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
3. The sodium pump needs its beta subunit.  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
4. Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
5. A proteolipid associated with Na,K-ATPase is not essential for ATPase activity.  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
6. Functional significance of the beta-subunit for heterodimeric P-type ATPases.  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
7. Na+,K(+)-ATPase.  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
8. Subunit requirements for expression of functional sodium pumps in yeast cells.  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
9. The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit.  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
10. Expression, targeting, and assembly of functional Na,K-ATPase polypeptides in baculovirus-infected insect cells.  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
11. Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
12. Structure and function of the Na,K pump: ten years of molecular biology.  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6) has 206 citations in a variety of scientific publications.

Primary antibodies like Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6) Specifications

Alternate Product Name: ATP1A1

Product Clone Name: C464.6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Na+/K+-ATPase α1

Epitope Species: rabbit

Product Reactivity/Detection: Na⁺/K⁺-ATPase α1

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 206 citations in various scientific publications

Available Conjugates/Forms for alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin