N-WASP Antibody (93-W) recommended for detection of N-WASP of mouse, rat and human origin by WB, IP, IF and ELISA

N-WASP Antibody (93-W): sc-100964

N-WASP Antibody (93-W) is rated 4.0 out of 5 by 3.
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Datasheets

    • N-WASP Antibody (93-W) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 100 µg/ml
    • raised against recombinant N-WASP of human origin
    • recommended for detection of N-WASP of mouse, rat and human origin by WB, IP, IF and ELISA
    • See WASP (B-9): sc-13139 for N-WASP antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using N-WASP (93-W): sc-100964 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced wonderful immunofluorescence nuclear Produced wonderful immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-16
Rated 4 out of 5 by from WB: crisp WB: crisp, clean band in IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-03-02
Rated 4 out of 5 by from Works well for western blotting with mouse N Works well for western blotting with mouse N-WASP transfected 293T and U-87 MG whole cell lysates. -SCBT QC
Date published: 2015-01-23
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