N-Myc Antibody (B8.4.B): sc-53993

N-Myc Antibody (B8.4.B) is a mouse monoclonal IgG2a kappa light chain antibody that detects N-Myc protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-N-Myc antibody (B8.4.B) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. N-Myc protein, a member of the Myc family of transcription factors, plays a crucial role in cell proliferation, differentiation, and apoptosis, making N-Myc a significant player in oncogenesis. N-Myc dysregulation, particularly through gene amplification, is commonly associated with aggressive forms of neuroblastoma, where N-Myc expression levels correlate with disease progression and patient prognosis. N-Myc protein is primarily located in the nucleus, where N-Myc functions as a transcriptional regulator, binding to specific DNA sequences to modulate the expression of target genes involved in cell cycle control and growth. Understanding N-Myc localization and function is vital for developing targeted therapies aimed at inhibiting N-Myc activity in cancer cells, thereby offering potential avenues for treatment in N-Myc-driven malignancies.

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N-Myc Antibody (B8.4.B) References:

1. MAZ, a zinc finger protein, binds to c-MYC and C2 gene sequences regulating transcriptional initiation and termination.  |  Bossone, SA., et al. 1992. Proc Natl Acad Sci U S A. 89: 7452-6. PMID: 1502157
2. Role of Src family kinases and N-Myc in spermatogonial stem cell proliferation.  |  Braydich-Stolle, L., et al. 2007. Dev Biol. 304: 34-45. PMID: 17222400
3. N-MYC regulates focal adhesion kinase expression in human neuroblastoma.  |  Beierle, EA., et al. 2007. J Biol Chem. 282: 12503-16. PMID: 17327229
4. Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation.  |  Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
5. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc.  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
6. The myc oncogene: its role in transformation and differentiation.  |  Cole, MD. 1986. Annu Rev Genet. 20: 361-84. PMID: 3028245
7. N-Myc-induced metabolic rewiring creates novel therapeutic vulnerabilities in neuroblastoma.  |  Tjaden, B., et al. 2020. Sci Rep. 10: 7157. PMID: 32346009
8. N‑Myc induces the tumor progression of prostate cancer by regulating FSCN1.  |  He, G., et al. 2020. Oncol Rep. 44: 2265-2274. PMID: 33000273
9. PHA-680626 Is an Effective Inhibitor of the Interaction between Aurora-A and N-Myc.  |  Boi, D., et al. 2021. Int J Mol Sci. 22: PMID: 34884931
10. N-Myc and STAT Interactor is an Endometriosis Suppressor.  |  Park, Y., et al. 2024. Int J Mol Sci. 25: PMID: 39125716
11. Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage.  |  Brodeur, GM., et al. 1984. Science. 224: 1121-4. PMID: 6719137
12. Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour.  |  Schwab, M., et al. Nature. 305: 245-8. PMID: 6888561

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-N-Myc Antibody (B8.4.B) has 209 citations in a variety of scientific publications.

Primary antibodies like Anti-N-Myc Antibody (B8.4.B) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

N-Myc Antibody (B8.4.B) Specifications

Alternate Product Name: MYCN

Product Clone Name: B8.4.B

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: N-Myc

Epitope Species: human

Product Reactivity/Detection: N-Myc

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 209 citations in various scientific publications

Available Conjugates/Forms for N-Myc Antibody (B8.4.B): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin