N-CoR Double Nickase Plasmid (m): sc-422771-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- N-CoR Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- N-CoR Double Nickase Plasmid (m) and N-CoR Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ncor1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: N-CoR Antibody (F-1): sc-515934
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-CoR Double Nickase Plasmid (m) | sc-422771-NIC | 20 µg | $410.00 |
Mouse Ncor1 encodes nuclear receptor corepressor 1 (N-CoR), a scaffold protein that assembles transcriptional repression complexes with HDAC3 and other cofactors to regulate chromatin accessibility and gene expression programs. N-CoR modulates signaling downstream of nuclear receptors and lineage-defining transcription factors, influencing cell fate decisions, metabolic homeostasis, and immune and inflammatory gene networks. Through epigenetic control of enhancer and promoter activity, N-CoR contributes to coordinated regulation of differentiation, circadian and hormonal responses, and stress-adaptive transcriptional states. Dysregulated Ncor1/N-CoR function has been implicated in pathways relevant to neurodevelopment, metabolism, and immune-associated phenotypes, making it a useful node for mechanistic studies in mouse models.
N-CoR Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ncor1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ncor1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ncor1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ncor1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.