N-cadherin Antibody (D-4): sc-8424

N-cadherin Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects N-cadherin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). N-cadherin monoclonal antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. N-cadherin, also known as CD325 or CDH2, plays a crucial role in cell-cell adhesion, essential for maintaining tissue integrity and facilitating morphogenetic processes during development. N-cadherin is primarily located at the cell membrane, forming adherens junctions that connect adjacent cells, thereby contributing to tissue structural stability. Such localization allows N-cadherin to interact with the cytoskeleton and other signaling molecules, influencing cellular behavior and communication. The extracellular domain of N-cadherin contains five homologous repeats mediating homophilic binding, while the intracellular domain interacts with cytoplasmic proteins like β-catenin, linking to the actin cytoskeleton and regulating cell motility and tissue architecture. Understanding N-cadherin function and localization helps elucidate involvement in various physiological processes and implications in diseases like cancer, where altered expression can lead to increased cell migration and metastasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-N-cadherin Antibody (D-4) has 121 citations in a variety of scientific publications.

Primary antibodies like Anti-N-cadherin Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

N-cadherin Antibody (D-4) Specifications

Alternate Product Name: CDH2

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 450-512

Epitope Target: N-cadherin

Epitope Species: human

Product Reactivity/Detection: N-cadherin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 121 citations in various scientific publications

Available Conjugates/Forms for N-cadherin Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred N-cadherin antibody is N-cadherin Antibody (13A9): sc-59987.