N-cadherin Antibody (D-4) recommended for detection of N-cadherin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

N-cadherin Antibody (D-4): sc-8424

N-cadherin Antibody (D-4) is rated 4.1 out of 5 by 10.
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Datasheets

    • Anti-N-cadherin Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) N-cadherin antibody provided at 200 µg/ml
    • raised against amino acids 450-512 within the extracellular domain of N-cadherin of human origin
    • Anti-N-cadherin Antibody (D-4) is recommended for detection of N-cadherin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
    • Anti-N-cadherin Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of N-cadherin (D-4): sc-8424.
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Can N-cadherin Antibody (D-4): sc-8424 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, N-cadherin Antibody (D-4): sc-8424 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 14
Date published: 2017-01-09
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Rated 4 out of 5 by from Good product It is good antibodies in WB. It works in dilution 1:1000.
Date published: 2018-07-14
Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 1 out of 5 by from Antibody does not work for IHC-P I purchased this antibody based on very nice images on the website showing specifically localization of NCAD in IHC-P. I tested the antibody using multiple techniques and tissue types with no staining. I reported the problems to the company and they kindly provided a replacement vial. Unfortunately the replacement did not work either again using multiple techniques and tissue types. While this antibody may have previously worked well in IHC-P, the current product being provided does not work for IHC-P.
Date published: 2018-02-03
Rated 1 out of 5 by from Almost no detectable in SK-hep1, RPMI8226 cell lin Almost no detectable in SK-hep1 and RPMI8226 cell lines. So I used femto. I only could detect strong background.
Date published: 2017-12-06
Rated 5 out of 5 by from Outstanding IHC !! Very clear and specific IHC staining data; also strong WB
Date published: 2017-01-11
Rated 5 out of 5 by from Very Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, equine, bovine, and human samples.
Date published: 2016-12-29
Rated 5 out of 5 by from Worked with fusion protien by WB with some Worked with fusion protien by WB with some background -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Very good antibody for IHC in paraffin Very good antibody for IHC in paraffin-embedded human sections. -SCBT QC
Date published: 2015-02-03
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